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Title:
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The death receptor antagonist FAIM promotes neurite outgrowth by a mechanism that depends on ERK and NF-κB signaling
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Author:
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Solé Serra, Carme; Dolcet Roca, Xavier; Segura Ginard, Miguel Francisco; Gutierrez, Humberto; Diaz Meco, Maria Teresa; Gozzelino, Raffaella; Sanchis, Daniel; Bayascas Ramírez, José Ramón; Gallego, Carme; Moscat, Jorge; Davies, Alun M.; Comella i Carnicé, Joan Xavier
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Notes:
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Fas apoptosis inhibitory molecule (FAIM) is a protein identified as an antagonist of Fas-induced cell death. We show that FAIM overexpression fails to rescue neurons from trophic factor deprivation, but exerts a marked neurite growth–promoting action in different neuronal systems. Whereas FAIM overexpression greatly enhanced neurite outgrowth from PC12 cells and sympathetic neurons grown with nerve growth factor (NGF), reduction of endogenous FAIM levels by RNAi decreased neurite outgrowth in these cells. FAIM overexpression promoted NF-κB activation, and blocking this activation by using a super-repressor IκBα or by carrying out experiments using cortical neurons from mice that lack the p65 NF-κB subunit prevented FAIM-induced neurite outgrowth. The effect of FAIM on neurite outgrowth was also blocked by inhibition of the Ras–ERK pathway. Finally, we show that FAIM interacts with both Trk and p75 neurotrophin receptor NGF receptors in a ligand-dependent manner. These results reveal a new function of FAIM in promoting neurite outgrowth by a mechanism involving activation of the Ras–ERK pathway and NF-κB. |
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Subject(s):
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-FAIM
-Neurobiologia
-Factors de creixement
-Receptors neurals
-Receptors cel·lulars
-Proteïnes
-Apoptosi
-Mort cel·lular |
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Rights:
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(c) Rockefeller University Press, 2004
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Document type:
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article
publishedVersion |
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Published by:
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Rockefeller University Press
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Share:
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