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Título:
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A Non-digestible Fraction of the Common Bean (Phaseolus vulgaris L.) Induces Cell Cycle Arrest and Apoptosis During Early Carcinogenesis
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Autor/a:
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Feregrino-Perez AA; Piñol Felis, Carme; Gómez Arbonés, Javier; Guevara-González RG; Campos-Vega R; Acosta-Gallegos J; Loarca-Piña G
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Notas:
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We have previously demonstrated that the non-digestible fraction (NDF) from common cooked beans (P. vulgaris L., cv Negro 8025) inhibits azoxymethane (AOM) induced colon cancer and influences the expression of genes involved in the induction of apoptosis and cell cycle
arrest through the action of butyrate. The objective of this study was to identify cell cycle alterations and morphological changes induced by treatment with AOM and to examine the formation of colonic aberrant crypt foci (ACF) in male Sprague Dawley rats fed with these beans. Rats were fed control diets upon arrival and were randomly placed into four groups after one week of acclimatization: control, NDF (intragastric administration), NDF+AOM and AOM. Rats treated with NDF+AOM exhibited a significantly lower number of total colonic ACF with a notable increase in the number of cells present in the G1 phase (83.14 %); a decreased proliferation index was observed in the NDF+AOM group
when compared to AOM group. NDF+AOM also displayed a higher number of apoptotic cells compared to AOM group. NDF of cooked common beans inhibited colon carcinogenesis at an early stage by inducing cell cycle arrest ofcolon cells and
morphological changes linked to apoptosis, thus confirming previous results obtained with gene expression studies. |
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Materia(s):
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-Non-digestible fraction
-Phaseolus vulgaris L.
-Flow cytometry
-Apoptosis
-Aberrant crypt foci
-Apoptosi
-Carcinogènesi
-Citometria de fluxe
-Apoptosis
-Carcinogenesis
-Flow cytometry |
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Derechos:
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(c) Springer Science Business Media, 2014
info:eu-repo/semantics/restrictedAccess |
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Tipo de documento:
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Artículo
Artículo - Versión publicada |
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Editor:
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Springer
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Compartir:
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