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Título:
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Comprehensive transcriptional analysis of the oxidative response in yeast
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Autor/a:
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Molina Navarro, Maria Micaela; Castells i Roca, Laia; Bellí i Martínez, Gemma; García-Martínez, José; Marín-Navarro, Julia; Moreno, Joaquín; Pérez-Ortín, José E.; Herrero Perpiñán, Enrique
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Notas:
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The oxidative stress response in Saccharomyces cerevisiae has
been analyzed by parallel determination of mRNA levels and
transcription rates for the entire genome. A mathematical algorithm
has been adapted for a dynamic situation such as the
response to stress, to calculate theoretical mRNA decay rates
from the experimental data. Yeast genes have been grouped into
25 clusters according to mRNA level and transcription rate
kinetics, and average mRNA decay rates have been calculated
for each cluster. In most of the genes, changes in one or both
experimentally determined parameters occur during the stress
response. 24% of the genes are transcriptionally induced without
an increase inmRNAlevels. The lack of parallelism between
the evolution of the mRNA amount and transcription rate predicts
changes inmRNAstability during stress. Genes for ribosomal
proteins and rRNA processing enzymes are abundant
among those whose mRNAs are predicted to destabilize. The
number of genes whose mRNAs are predicted to stabilize is
lower, although some protein folding or proteasomal genes are
among the latter. We have confirmed the mathematical predictions
for several genes pertaining to different clusters by experimentally
determining mRNA decay rates using the regulatable
tetO promoter in transcriptional expression conditions not
affected by the oxidative stress. This study indicates that the
oxidative stress response in yeast cells is not only conditioned by
gene transcription but also by the mRNA decay dynamics and
that this complex response may be particularly relevant to
explain the temporary down-regulation of protein synthesis
occurring during stress. |
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Derechos:
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(c) American Society for Biochemistry and Molecular Biology, 2008
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Tipo de documento:
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article
publishedVersion |
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Editor:
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The American Society for Biochemistry and Molecular Biology
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