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Title:
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The oxidative stress response in yeast cells involves changes in the stability of Aft1 regulon mRNAs
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Author:
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Castells i Roca, Laia; Mühlenhoff, Ulrich; Lill, Roland; Herrero Perpiñán, Enrique; Bellí i Martínez, Gemma
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Notes:
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Saccharomyces cerevisiae can import iron through
a high-affinity system consisting of the Ftr1/Fet3-
mediated reductive pathway and the siderophoremediated
non-reductive one. Expression of
components of the high-affinity system is controlled
by the Aft1 transcriptional factor. In this study we
show that, upon oxidative stress, Aft1 is transitorily
internalized into the nucleus, followed by transcription
activation of components of its regulon. In these
conditions, the mRNA levels of the genes of the nonreductive
pathway become increased, while those of
FTR1 and FET3 remain low because of destabilization
of the mRNAs. Consequently, the respective protein
levels also remain low. Such mRNA destabilization is
mediated by the general 5 –3 mRNA decay pathway
and is independent of the RNA binding protein Cth2.
Yeast cells are hypersensitive to peroxides in growth
conditions where only the high-affinity reductive
pathway is functional for iron assimilation. On the
contrary, peroxide does not affect growth when iron
uptake occurs exclusively through the non-reductive
pathway. This reinforces the idea that upon oxidative
stress S. cerevisiae cells redirect iron assimilation
through the non-reductive pathway to minimize oxidative
damage by the ferrous ions, which are formed
during iron import through the Ftr1/Fet3 complexes. |
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Rights:
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(c) John Wiley & Sons, 2011
(c) Blackwell Publishing, 2011
info:eu-repo/semantics/restrictedAccess |
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Document type:
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article
publishedVersion |
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Published by:
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John Wiley & Sons;
Blackwell Publishing
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