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Title:
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Isolation of a Candida albicans gene, tightly linked to URA3, coding for a putative transcription factor that suppresses a Saccharomyces cerevisiae aft1 mutation
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Author:
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Gómez García, Micaela; O'Connor, José-Enrique; Latorre García, Lorena; Irar Martínez, Sami; Herrero Perpiñán, Enrique; Del Castillo Agudo, Lucas
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Notes:
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A pathogen such as C. albicans needs an ef®cient mechanism of iron uptake in an iron-
restricted environment such as is the human body. A ferric-reductase activity regulated by
iron and copper, and analogous to that in S. cerevisiae, has been described in C. albicans.
We have developed an in-plate protocol for the isolation of clones that complement an aft1
mutation in S. cerevisiae that makes cells dependent on iron for growth. After
transformation of S. cerevisiae aft1 with a C. albicans library, we have selected clones
that grow in conditions of iron de®ciency and share an identical plasmid, pIRO1, with a
4500 bp insert containing the URA3 gene and an ORF (IRO1) responsible for the
suppression of the iron dependency. IRO1 does not show homology with AFT1 or with
other sequences in the databases. Northern analysis demonstrates constitutive expression
of IRO1. CAI4, a C. albicans strain isolated as Dura3, also has a deletion of the 3k half of
IRO1, and displays in YNB medium similar phenotypic characteristics to S. cerevisiae aft1
mutant strains. Therefore, we consider IRO1 as a gene of C. albicans involved in the
utilization of iron. However, in extreme conditions of iron deprivation, CAI4 seems to
activate alternative mechanisms of iron uptake that allow a better growth than the wild
strain SC5314. Analysis of its predicted protein sequence is in agreement with a role of
Iro1p as a transcription factor. |
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Subject(s):
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-Candida albicans
-Iron uptake
-Transcription factor |
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Rights:
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(c) John Wiley & Sons, 2001
info:eu-repo/semantics/restrictedAccess |
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Document type:
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article
publishedVersion |
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Published by:
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John Wiley & Sons
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