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Title:
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Prokaryotic and eukaryotic monothiol glutaredoxins are able to perform the functions of Grx5 in the biogenesis of Fe/S clusters in yeast mitochondria
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Author:
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Molina Navarro, Maria Micaela; Casas Herranz, Celia; Piedrafita Llorens, Lídia; Bellí i Martínez, Gemma; Herrero Perpiñán, Enrique
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Notes:
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The Saccharomyces cerevisiae monothiol glutare-
doxin Grx5 participates in the mitochondrial biogenesis of
iron–sulfur clusters. Grx5 homologues exist in organisms from
bacteria to humans. Chicken (cGRX5) and human (hGRX5)
homologues contain a mitochondrial targeting sequence, suggest-
ing a mitochondrial localization for these two proteins. We have
compartmentalized the Escherichia coli and Synechocystis sp.
homologues, and also cGRX5 and hGRX5, in the mitochondrial
matrix of a yeast grx5 mutant. All four heterologous proteins
rescue the defects of the mutant. The chicken cGRX5 gene was
significantly expressed throughout the embryo stages in different
tissues. These results underline the functional conservation of
Grx5 homologues throughout evolution. |
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Subject(s):
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-Glutaredoxin
-Iron–sulfur cluster
-Mitochondria
-Redox regulation
-Glutathione |
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Rights:
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(c) Federation of European Biochemical Societies, 2006
(c) Elsevier, 2006
info:eu-repo/semantics/restrictedAccess |
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Document type:
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article
publishedVersion |
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Published by:
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Elsevier
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