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Title:
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Caloric restriction reveals a lipidomic and metabolomic signature in liver of male mice
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Author:
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Jové Font, Mariona; Naudí i Farré, Alba; Ramírez-Núñez, Omar; Portero Otín, Manuel; Selman, Colin; Withers, Dominic J.; Pamplona Gras, Reinald
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Notes:
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Lipid composition, particularly membrane unsaturation, has been proposed as being a lifespan determinant, but it is currently unknown whether caloric restriction (CR), an accepted life-extending intervention, affects cellular lipid profiles. In this study, we employ a liquid chromatography quadrupole time-of-flight-based methodology to demonstrate that CR in the liver of male C57BL/6 mice: (i) induces marked changes in the cellular lipidome, (ii) specifically reduces levels of a phospholipid peroxidation product, 1-palmitoyl-2-glutaryl-sn-glycero-3-phosphatidylcholine, (iii) alters cellular phosphoethanolamine and triglyceride distributional profiles, (iv) affects mitochondrial electron transport chain complexes, increasing complex II and decreasing complex III and (v) is associated with specific changes in liver metabolic pathways. These data demonstrate that CR induces a specific lipidome and metabolome reprogramming event in mouse liver which is associated with lower protein oxidative damage, as assessed by mass spectrometry-based measurements. Such changes may be critical to the increased lifespan and healthspan observed in C57BL/6 mice following CR. |
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Subject(s):
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-Metabolisme dels lípids
-Malalties del fetge
-Lipid metabolism
-Liver diseases |
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Rights:
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cc-by (c) Jové Font, Mariona et al., 2014
http://creativecommons.org/licenses/by/3.0/es
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Document type:
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Article
Article - Published version |
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Published by:
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John Wiley & Sons;
Anatomical Society
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