Título:
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Comparative analysis of hemolymph proteome maps in diapausing and non-diapausing larvae of Sesamia nonagrioides
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Autor/a:
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Pérez Hedo, Meritxell; Sanchez Lopez, Isabel; Eizaguirre Altuna, Matilde
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Notas:
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Background: Sesamia nonagrioides is a noctuid that feeds on maize, sugar cane and sorghum in North Africa and
Southern Europe. Larvae reared under long day conditions pupate after 5 or 6 larval instars, whereas larvae reared
under short day conditions enter diapause and undergo up to 12 molts before dying or pupating. To better
understand the mechanism of larval development and diapause, we identified proteins with different expressions in
the sixth instar of diapausing and non-diapausing larvae.
Results: A total of 52 differentially regulated proteins were detected in the hemolymph of the diapausing or
non-diapausing larvae at the beginning or end of the sixth instar. From these proteins, 11 were identified by mass
spectrometry (MALDI-TOF MS or MALDI-TOF/TOF MS/MS): 5 were upregulated in the hemolymph of
non-diapausing larvae and 6 in the hemolymph of the diapausing larvae. Interestingly, some proteins were
expressed only in non-diapausing larvae but none was expressed only in the hemolymph of diapausing larvae. The
possible functions of some of these proteins related to diapause maintenance or to larval-pupal metamorphosis are
discussed.
Conclusions: The 2-DE proteomic map of S. nonagrioides hemolymph shows differential protein expression in
diapausing and non-diapausing larvae. Some proteins that showed higher expression in the diapausing larvae at
the end of the sixth instar could be involved in JH level maintenance thus in the diapause status maintenance. On
the contrary, other proteins that showed the highest expression or that were expressed only in the non-diapausing
larvae could be involved in larval-pupal metamorphosis.
This study was supported by the Spanish R&D Agency (Comisión
Interministerial de Ciencia y Tecnología) through project AGL2011-23996. |
Materia(s):
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-Diapause -Proteomic analysis -Hemolymph |
Derechos:
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cc-by (c) Pérez Hedo, Meritxell et al., 2012
http://creativecommons.org/licenses/by/3.0/es/
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Tipo de documento:
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article publishedVersion |
Editor:
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BioMed Central
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Compartir:
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