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Título:
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Cth2 protein mediates early adaptation of yeast cells to oxidative stress conditions
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Autor/a:
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Castells i Roca, Laia; Pijuan Marquilles, Jordi; Ferrezuelo, Francisco; Bellí i Martínez, Gemma; Herrero Perpiñán, Enrique
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Notas:
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Cth2 is an mRNA-binding protein that participates in remodeling yeast cell metabolism in
iron starvation conditions by promoting decay of the targeted molecules, in order to avoid
excess iron consumption. This study shows that in the absence of Cth2 immediate upregulation
of expression of several of the iron regulon genes (involved in high affinity iron uptake
and intracellular iron redistribution) upon oxidative stress by hydroperoxide is more intense
than in wild type conditions where Cth2 is present. The oxidative stress provokes a temporary
increase in the levels of Cth2 (itself a member of the iron regulon). In such conditions
Cth2 molecules accumulate at P bodies-like structures when the constitutive mRNA decay
machinery is compromised. In addition, a null Δcth2 mutant shows defects, in comparison
to CTH2 wild type cells, in exit from α factor-induced arrest at the G1 stage of the cell cycle
when hydroperoxide treatment is applied. The cell cycle defects are rescued in conditions
that compromise uptake of external iron into the cytosol. The observations support a role of
Cth2 in modulating expression of diverse iron regulon genes, excluding those specifically
involved in the reductive branch of the high-affinity transport. This would result in immediate
adaptation of the yeast cells to an oxidative stress, by controlling uptake of oxidant-promoting
iron cations.
This study was supported by grant BFU2010-17656 from Spanish Ministerio de Economía y Competitividad (www.mineco.gob.es). |
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Derechos:
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cc-by, (c) Castells-Roca et al., 2016
http://creativecommons.org/licenses/by/3.0/es/
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Tipo de documento:
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article
publishedVersion |
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Editor:
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Public Library of Science
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Compartir:
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