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Título:
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Functional characterization of the Gentiana lutea zeaxanthin epoxidase (GlZEP) promoter in transgenic tomato plants
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Autor/a:
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Yang, Qingjie; Yuan, Dawei; Shi, Lianxuan; Capell Capell, Teresa; Bai, Chao; Wen, Nuan; Lu, Xiaodan; Sandmann, Gerhard; Christou, Paul; Zhu, Changfu
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Notas:
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The accumulation of carotenoids in plants
depends critically on the spatiotemporal expression
profiles of the genes encoding enzymes in the carotenogenic
pathway. We cloned and characterized the
Gentiana lutea zeaxanthin epoxidase (GlZEP) promoter
to determine its role in the regulation of
carotenogenesis, because the native gene is expressed
at high levels in petals, which contain abundant
chromoplasts. We transformed tomato (Solanum
lycopersicum cv. Micro-Tom) plants with the gusA
gene encoding the reporter enzyme b-glucuronidase
(GUS) under the control of the GlZEP promoter, and
investigated the reporter expression profile at the
mRNA and protein levels. We detected high levels of
gusA expression and GUS activity in chromoplastcontaining
flowers and fruits, but minimal levels in
immature fruits containing green chloroplasts, in
sepals, leaves, stems and roots. GlZEP-gusA expression
was strictly associated with fruit development and
chromoplast differentiation, suggesting an evolutionarily-conserved
link between ZEP and the differentiation
of organelles that store carotenoid pigments. The
impact of our results on current models for the
regulation of carotenogenesis in plants is discussed.
Our work was supported by grants from the Spanish Ministry of Science and Innovation (MICINN) (BFU2007-61413); European Research Council Advanced Grant (BIOFORCE) to PC; Aciones complementarias, BIO2007-30738-E MICINN, Spain, grants from the National Natural Science Foundation of China (grant nos. 31070269, 30370123, 39970069) and the Programme for Introducing Talents to Universities (B07017). |
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Materia(s):
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-Gentiana lutea
-Zeaxanthin epoxidase
-Promoter
-b-Glucuronidase |
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Derechos:
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(c) Springer Science Business Media B.V., 2012
info:eu-repo/semantics/restrictedAccess |
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Tipo de documento:
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article
publishedVersion |
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Editor:
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Springer Verlag
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