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Title:
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Targeted transcriptomic and metabolic profiling reveals temporal bottlenecks in the maize carotenoid pathway that may be addressed by multigene engineering
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Author:
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Farré Martinez, Gemma; Rivera Vélez, Sol Maiam; Alves, Rui; Vilaprinyo Terré, Ester; Sorribas Tello, Albert; Canela i Garayoa, Ramon; Naqvi, Shaista; Sandmann, Gerhard; Capell Capell, Teresa; Zhu, Changfu; Christou, Paul
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Notes:
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Carotenoids are a diverse group of tetraterpenoid pigments found in plants, fungi, bacteria and some animals.
They play vital roles in plants and provide important health benefits to mammals, including humans. We previously
reported the creation of a diverse population of transgenic maize plants expressing various
carotenogenic gene combinations and exhibiting distinct metabolic phenotypes. Here we performed an
in-depth targeted mRNA and metabolomic analysis of the pathway to characterize the specific impact of five
carotenogenic transgenes and their interactions with 12 endogenous genes in four transgenic lines representing
distinct genotypes and phenotypes. We reconstructed the temporal profile of the carotenoid pathway
during endosperm development at the mRNA and metabolic levels (for total and individual carotenoids), and
investigated the impact of transgene expression on the endogenous pathway. These studies enabled us to
investigate the extent of any interactions between the introduced transgenic and native partial carotenoid
pathways during maize endosperm development. Importantly, we developed a theoretical model that
explains these interactions, and our results suggest genetic intervention points that may allow the maize
endosperm carotenoid pathway to be engineered in a more effective and predictable manner.
This study was supported by the Ministerio de Ciencia e Innovación, Spain (BIO2007-61413, BIO2011-22525 and PM2010PKB-00746 CAROMAIZE) and a European Research Council Advanced Grant (BIOFORCE) to P.C. G.F. is the recipient of a PhD fellowship from the Ministerio de Ciencia e Innovacion, Spain (BES-2008-003894). R.A. and A.S. are partially funded through Research Group 2009SGR809 from the Generalitat de Catalunya and through grants BFU2008-0196 to A.S. and BFU2010-17704 to R.A., both from the Ministerio de Ciencia e Innovacion, Spain. |
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Subject(s):
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-Metabolic engineering
-Zea mays
-Carotenoids
-Ketocarotenoids |
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Rights:
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(c) els autors, 2013
(c) Wiley, 2013
info:eu-repo/semantics/restrictedAccess |
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Document type:
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article
publishedVersion |
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Published by:
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Wiley
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Share:
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