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Títol:
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Regulation of the Na+/K+-ATPase Ena1 Expression by Calcineurin/Crz1 under High pH Stress: a quantitative study
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Autor/a:
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Petrezsélyová, Silvia; López Malo, María; Canadell, David; Roque, Alicia; Serra Cardona, Albert; Marqués, M. Carmen; Vilaprinyo Terré, Ester; Alves, Rui; Yenush, Lynne; Ariño Carmona, Joaquín
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Notes:
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Regulated expression of the Ena1 Na+-ATPase is a crucial event for adaptation to high salt
and/or alkaline pH stress in the budding yeast Saccharomyces cerevisiae. ENA1 expression
is under the control of diverse signaling pathways, including that mediated by the calciumregulatable
protein phosphatase calcineurin and its downstream transcription factor Crz1.
We present here a quantitative study of the expression of Ena1 in response to alkalinization
of the environment and we analyze the contribution of Crz1 to this response. Experimental
data and mathematical models substantiate the existence of two stress-responsive Crz1-
binding sites in the ENA1 promoter and estimate that the contribution of Crz1 to the early
response of the ENA1 promoter is about 60%. The models suggest the existence of a second
input with similar kinetics, which would be likely mediated by high pH-induced activation
of the Snf1 kinase.
This work was supported by grants BFU2011-30197-C3-01, BFU2014-54591-C2-1-P and EUI2009-04147 (SysMo2) to JA. (Ministry of Industry and Competitivity, Spain, and Fondo Europeo de Desarrollo Regional [FEDER]). JA is the recipient of an Ajut 2014SGR-4 award (Generalitat de Catalunya). DC was recipient of a predoctoral fellowship from the Spanish Ministry of Education. |
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Drets:
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cc-by (c) Petrezsélyová et al., 2016
http://creativecommons.org/licenses/by/3.0/es/
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Tipus de document:
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article
publishedVersion |
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Publicat per:
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Public Library of Science
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