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Títol:
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The silencing of carotenoid β-hydroxylases by RNA interference in different maize genetic backgrounds increases the β-carotene content of the endosperm
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Autor/a:
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Berman Quintana, Judit; Zorrilla López, Uxue; Sandmann, Gerhard; Capell Capell, Teresa; Christou, Paul; Zhu, Changfu
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Notes:
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Maize (Zea mays L.) is a staple food in many parts of Africa, but the endosperm generally
contains low levels of the pro-vitamin A carotenoid -carotene, leading to vitamin A deficiency
disease in populations relying on cereal-based diets. However, maize endosperm does accumulate
high levels of other carotenoids, including zeaxanthin, which is derived from -carotene via two
hydroxylation reactions. Blocking these reactions could therefore improve the endosperm -carotene
content. Accordingly, we used RNA interference (RNAi) to silence the endogenous ZmBCH1 and
ZmBCH2 genes, which encode two non-heme di-iron carotenoid -hydroxylases. The genes were
silenced in a range of maize genetic backgrounds by introgressing the RNAi cassette, allowing
us to determine the impact of ZmBCH1/ZmBCH2 silencing in diverse hybrids. The -carotene
content of the endosperm increased substantially in all hybrids in which ZmBCH2 was silenced,
regardless of whether or not ZmBCH1 was silenced simultaneously. However, the -carotene content
did not change significantly in C17 hybrids (M7 C17 and M13 C17) compared to C17 alone,
because ZmBCH2 is already expressed at negligible levels in the C17 parent. Our data indicate that
ZmBCH2 is primarily responsible for the conversion of -carotene to zeaxanthin in maize endosperm |
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Matèries:
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-Maize (Zea mays L.)
-RNAi
-Hybrid |
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Drets:
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cc-by, (c) Berman et al., 2017
https://creativecommons.org/licenses/by/4.0/
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Tipus de document:
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article
publishedVersion |
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Publicat per:
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MDPI
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Compartir:
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