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Título:
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Study of Merkel cells in the dog through the immunohistochemical expression of five different commercial antibodies: comparative analysis
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Autor/a:
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Ramírez, Gustavo A.; Rodríguez, Francisco; Suárez-Bonnet, Alejandro; Herráez, Pedro; Castro-Alonso, Ayoze; Rivero, Miguel; Espinosa de los Monteros, Antonio
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Notas:
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Merkel cells (MCs) are cutaneous specialized cells with a wide immunohistochemical profile, including low
molecular weight cytokeratins and various endocrine and neural markers. Differences in expression of
these markers may be a consequence of contradictory results in studies of MCs in normal and damaged
canine tissues. The present study aimed to compare five different commercial available antibodies
developed against cytokeratins 8 + 18 and 20, neuron-specific enolase, chromogranin A, and
synaptophysin on formalin-fixed paraffin-embedded samples of hard palate, cheek skin including sinus
hair follicles or vibrissae, nasal planum, and footpads. The antibodies showed great variability with respect
to quality and intensity of immunoreactivity to identify MCs. Anti-Cytokeratin 20 antibody is more effective
to recognize MCs in the dog skin followed by the antibodies against neuron-specific enolase, cytokeratins
8 and18, chromogranin A, and synaptophysin. There was also a significant difference in intensity of
immunoreaction scores between them depending on the location examined. These results represent a
necessary basic background for future studies of the role of these cells in normal and damaged canine tissues.
This study was supported by the Ministry of Education, Culture and Sport of the Government of Spain (Program for Development of University Pro- fessors and Researchers, BOE 12022000). |
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Materia(s):
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-Antibody
-Canine
-Comparative analysis
-Immunohistochemistry |
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Derechos:
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cc-by (c) Ramírez et al., 2017
http://creativecommons.org/licenses/by/4.0/
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Tipo de documento:
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article
publishedVersion |
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Editor:
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Taylor & Francis
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Compartir:
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