Title:
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Diacylglycerol is required for the formation of COPI vesicles in the Golgi-to-ER transport pathway
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Author:
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Sarri Plans, Elisabet; Martínez Rodríguez, Susana Eva; Jiménez, Nuria; Claro Izaguirre, Enrique; Mérida, Isabel; Burger, Koert N. J.; Egea Guri, Gustavo; Fernández-Ulibarri, Inés; Vilella, Montserrat; Lázaro-Diéguez, Francisco
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Other authors:
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Universitat de Barcelona |
Abstract:
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Diacylglycerol is necessary for trans-Golgi network (TGN) to cell surface transport, but its functional relevance in the early secretory pathway is unclear. Although depletion of diacylglycerol did not affect ER-to-Golgi transport, it led to a redistribution of the KDEL receptor to the Golgi, indicating that Golgi-to-ER transport was perturbed. Electron microscopy revealed an accumulation of COPI-coated membrane profiles close to the Golgi cisternae. Electron tomography showed that the majority of these membrane profiles originate from coated buds, indicating a block in membrane fission. Under these conditions the Golgi-associated pool of ARFGAP1 was reduced, but there was no effect on the binding of coatomer or the membrane fission protein CtBP3/BARS to the Golgi. The addition of 1,2-dioctanoyl-sn-glycerol or the diacylglycerol analogue phorbol 12,13-dibutyrate reversed the effects of endogenous diacylglycerol depletion. Our findings implicate diacylglycerol in the retrograde transport of proteins from Golgi to the ER and suggest that it plays a critical role at a late stage of COPI vesicle formation. |
Subject(s):
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-Aparell de Golgi -Reticle endoplasmàtic -Proteïnes -Citologia -Transport biològic -Golgi apparatus -Endoplasmic reticulum -Proteins -Cytology -Biological transport |
Rights:
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cc-by-nc-sa (c) Fernández-Ulibarri et al., 2007
http://creativecommons.org/licenses/by-nc-sa/3.0/ |
Document type:
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Article Article - Published version |
Published by:
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American Society for Cell Biology
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