Title:
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MicroRNAs expression, chromosomal alterations and immunoglobulin variable Heavy chain hypermutations in Mantle Cell Lymphomas
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Author:
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López-Guillermo, A; Navarro, A; Beà, Sílvia; Fernández, V; Prieto, M; Salaverria, I; Jares, P; Hartmann, E; Mozos, A; Hernández, L; Campo, E; Rossenwald, A; Serrano, Sergi; Solé Parellada, Francesc; Ott, G; Puig Oriol, Xavier; Colomer, D; Villamor, N.
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Other authors:
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Universitat Politècnica de Catalunya. Departament d'Estadística i Investigació Operativa; Universitat Politècnica de Catalunya. GRESA - Grup de recerca en estadística aplicada |
Abstract:
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The contribution of microRNAs (miR) to the pathogenesis of mantle cell lymphoma (MCL) is not well known.We investigated
the expression of 86 mature miRs mapped to frequently altered genomic regions in MCL in CD5+/CD5 normal B cells, reactive
lymph nodes, and purified tumor cells of 17 leukemic MCL, 12 nodal MCL, and 8MCL cell lines. Genomic alterations of the
tumors were studied by single nucleotide polymorphism arrays
and comparative genomic hybridization. Leukemic and nodal
tumors showed a high number of differentially expressed miRs
compared with purified normal B cells, but only some of them
were commonly deregulated in both tumor types. An unsupervised
analysis of miR expression profile in purified leukemic
MCL cells revealed two clusters of tumors characterized by
different mutational status of the immunoglobulin genes,
proliferation signature, and number of genomic alterations.
The expression of most miRs was not related to copy number
changes in their respective chromosomal loci. Only the levels
of miRs included in the miR-17-92 cluster were significantly
related to genetic alterations at 13q31. Moreover, overexpression
of miR-17-5p/miR-20a from this cluster was associated
with high MYC mRNA levels in tumors with a more aggressive
behavior. In conclusion, the miR expression pattern of MCL is
deregulated in comparison with normal lymphoid cells and
distinguishes two subgroups of tumors with different biological
features. |
Subject(s):
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-Àrees temàtiques de la UPC::Ciències de la salut::Medicina -Càncer -Limfomes -Cromosomes |
Rights:
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Attribution-NonCommercial-NoDerivs 3.0 Spain
http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
Document type:
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Article - Updated version Article |
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