Title:
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Individual-based model and simulation of Plasmodium falciparum infected erythrocyte in vitro cultures
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Author:
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Ferrer Savall, Jordi; Vidal, J.; Prats Soler, Clara; Valls Ribas, Joaquim; Herreros, E.; López Codina, Daniel; Giró Roca, Antoni; Gargallo-Viola, Domingo
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Other authors:
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Universitat Politècnica de Catalunya. Departament de Física i Enginyeria Nuclear; Universitat Politècnica de Catalunya. SC-SIMBIO - Sistemes complexos. Simulació discreta de materials i de sistemes biològics |
Abstract:
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Malaria is still one of the most fatal diseases in the world. Development of an effective treatment or vaccine requires the cultivation of the parasite that causes it: Plasmodium falciparum. Several methods for in vitro cultivation of P. falciparum infected erythrocytes have been successfully developed and described in the last 30 years. Some problems arising from the current harvests are the low parasitaemia
and daily human supervision requirements. The lack of a suitable model for global culture behavior makes the assay of new
methodologies a costly and tenuous task. In this paper we present a model and simulation tool for these systems. We use the INDividual
DIScrete SIMulation protocol (INDISIM) to qualitatively reproduce the temporal evolution of the erythrocyte and merozoite
populations. Whole system dynamics are inferred by setting the rules of behavior for each individual red blood cell, such as the nutrient uptake, metabolism and infection processes, as well as the properties and rules for the culture medium: composition, diffusion and external manipulation. We set the individual description parameters according to the values in published data, and allow population heterogeneity. Cells are arranged in a three-dimensional grid and the study is focused on the geometric constraints and physical design of experimental sets. Several published experimental cultures have been reproduced with computer simulations of this model, showing that the observed experimental behavior can be explained by means of individual interactions and statistical laws. |
Subject(s):
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-Àrees temàtiques de la UPC::Enginyeria agroalimentària::Ciències de la terra i de la vida::Microbiologia -In vitro -Malaria -Individual-based model
Plasmodium falciparum
Simulation
Merozoite
Erythrocyte
In vitro culture
Malaria -Cultiu in vitro -Malària |
Rights:
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Document type:
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Article - Published version Article |
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