Abstract:
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This master thesis contains experimental research about relative impact of
significant process parameters that influence the pomelo´s (Citrus maxima)
extraction using Taguchi design methodology. Orthogonal array L18 was selected
for the proposed design and ANOVA method was used for recognizing the relative
influence of the process parameters. Six different studies have been carried out:
To obtain a high yield of the pomelo extraction the most important parameters
were: Pomelo´s material, ratio between mass of material and volume of solvent
and type of extraction.
The content of total extracted phenols (according to the Folin-Ciocalteu procedure)
was analyzed by UV spectrophotometry. The most influential parameters were
Pomelo´s material and ratio between mass of material and volume of solvent.
Extracts were analyzed for the contents of naringin by high performance liquid
chromatography (HPLC). The analysis was performed by solubiliring the extracts
in methanol. The most important parameters for extraction of naringin were:
Pomelo´s material, ratio between mass of material and volume of solvent, time,
type of extraction and temperature.
For determination of antioxidant properties of extracts three different methods
were used.
The radical-scavenging activities of pomelo extract were evaluated using the
stable free radical 2,2-diphenyl-1-picrylhidrazyl (DPPH·). The most influential parameters were: temperature, pomelo’s material and kind of solvent.
The most influential parameters for antioxidant properties of extracts determined
by ACW method (water soluble antioxidant capacity) were: Pomelo´s material and
ratio between mass of material and volume of solvent, while for lipid soluble
antioxidant capacity (ACL) the most influential parameters were: time, solvent
percentage, pomelo´s material, type of extraction and kind of solvent. |