Títol:
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Protein phosphatase inhibition assays for okadaic acid detection in shellfish: matrix effects, applicability and comparison with LC-MS/MS analysis
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Autor/a:
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Garibo, Diana; Dàmaso, Esther; Eixarch Puigcerver, Helena; De la Iglesia González, Pablo de la; Fernández-Tejedor, Margarita; Diogène Fadini, Jorge; Pazos, Yolanda; Campàs i Homs, Mònica
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Altres autors:
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IRTA. Recerca i Tecnologia Agroalimentàries |
Abstract:
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The applicability of the protein phosphatase inhibition assay (PPIA) to the determination of okadaic acid
(OA) and its acyl derivatives in shellfish samples has been investigated, using a recombinant PP2A and a
commercial one. Mediterranean mussel, wedge clam, Pacific oyster and flat oyster have been chosen as
model species. Shellfish matrix loading limits for the PPIA have been established, according to the
shellfish species and the enzyme source. A synergistic inhibitory effect has been observed in the presence
of OA and shellfish matrix, which has been overcome by the application of a correction factor (0.48).
Finally, Mediterranean mussel samples obtained from Rı´a de Arousa during a DSP closure associated to
Dinophysis acuminata, determined as positive by the mouse bioassay, have been analysed with the PPIAs.
The OA equivalent contents provided by the PPIAs correlate satisfactorily with those obtained by liquid
chromatography–tandem mass spectrometry (LC–MS/MS). |
Data de publicació:
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22-02-2013 |
Matèries:
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Toxines marines Espectrometria de Mases Cromatografia Líquida |
Drets:
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L'accés als continguts d'aquest document queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
Pàgines:
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35 p. |
Tipus de document:
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Article Article - Esborrany |
Publicat per:
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Elsevier
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Compartir:
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