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Título:	Comparative gene finding in chicken indicates that we are closing in on the set of multi-exonic widely-expressed human genes
Autor/a:	Castelo Valdueza, Robert; Reymond, Alexandre; Wyss, Carine; Cámara, Francisco; Parra Farré, Genís; Antonarakis, Stylianos E.; Eyras Jiménez, Eduardo; Guigó Serra, Roderic
Otros autores:	Universitat Pompeu Fabra
Abstract:	The recent availability of the chicken genome sequence poses the question of whether there are human protein-coding genes conserved in chicken that are currently not included in the human gene catalog. Here, we show, using comparative gene finding followed by experimental verification of exon pairs by RT–PCR, that the addition to the multi-exonic subset of this catalog could be as little as 0.2%, suggesting that we may be closing in on the human gene set. Our protocol, however, has two shortcomings: (i) the bioinformatic screening of the predicted genes, applied to filter out false positives, cannot handle intronless genes; and (ii) the experimental verification could fail to identify expression at a specific developmental time. This highlights the importance of developing methods that could provide a reliable estimate of the number of these two types of genes.
Abstract:	This research is supported by grants BIO2000-1358-C02-02 from ‘Plan Nacional de I+D’ (Spain), Jérôme Lejeune, Childcare and Désirée and Niels Yde Foundations (Switzerland), the European Union Biosapiens Network of Excellence, the Swiss National Science Foundation and the NCCR Frontiers in Genetics. Funding to pay the Open Access publication charges for this article was provided by grant BIO2000-1358-C02-02 from ‘Plan Nacional de I+D’ (Spain).
Materia(s):	-Genomes -- Anàlisi -Biologia computacional -- Mètodes -Expressió gènica -Transcriptasa inversa -Animals -Exons -Humans -Genome -Chickens -Reverse Transcriptase -Computational Biology -Gene Expression
Derechos:	(c) The Author(s) 2005. Published by Oxford University Press. All rights reserved. The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed; the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given; if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative work this must be clearly indicated. For commercial re-use, please contact journals.permissions@oupjournals.org
Tipo de documento:	Artículo Artículo - Versión publicada
Editor:	Oxford University Press
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