Títol:
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Mineralization of DNA into nanoparticles of hydroxyapatite
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Autor/a:
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Bertran Cànovas, Òscar; Valle Mendoza, Luis Javier del; Revilla López, Guillermo; Chaves Barboza, Gustavo Adolfo; Cardús Andreu, Lluís; Casas Becerra, María Teresa; Casanovas Salas, Jordi; Turón Dols, Pau; Puiggalí Bellalta, Jordi; Alemán Llansó, Carlos
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Altres autors:
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Universitat Politècnica de Catalunya. Departament de Física Aplicada; Universitat Politècnica de Catalunya. Departament d'Enginyeria Química; Universitat Politècnica de Catalunya. IMEM - Innovació, Modelització i Enginyeria en (BIO) Materials; Universitat Politècnica de Catalunya. PSEP - Polimers Sintètics: Estructura i Propietats. Polimers Biodegradables. |
Abstract:
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Encapsulation of DNA into hydroxyapatite (HAp) has been investigated using a rational approach that involves computer simulation and experimental techniques. The temporal evolution of the radial distribution
functions derived from atomistic molecular dynamics simulations of Ca2+, PO4
3− and OH−-containing aqueous solutions in the presence and absence of B-DNA has been used to conclude that the backbone of the double helix acts as a template for HAp growth. More specifically, results reveal the formation of calcium phosphate clusters at the first stages of the simulations, which subsequently reorganize
to nucleate HAp. This effect is produced in the absence and, especially, presence, of DNA indicating that the biomolecules do not inhibit but even promote mineral growth. Furthermore, computer simulations suggest that the diffusion of the OH− anions through the inorganic solution is the limiting step
for the nucleation of the biomineral. Nanocapsules and crystalline nanorods of HAp containing DNA molecules inside have been prepared by mixing solutions containing Ca2+ and PO4 3− ions with fish sperm
DNA at high pH. The dimensions and morphology of such nanostructures have been examined by transmission electron microscopy, while the characterization of the biomineral has been focused on the
identification of DNA inside HAp using infrared, X-ray photoelectron and UV-vis spectroscopies, as well as gel electrophoresis. The biominerals reported in this work are important for biomedical applications requiring the protection of DNA from aggressive environmental conditions |
Matèries:
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-Àrees temàtiques de la UPC::Física -Hydroxyapatite -Biomolecules -Atomistic molecular dynamics simulations -Biomedical applications -Crystalline nanorods -Environmental conditions -Experimental techniques -Hydroxyapatite (HAp) -Radial distribution functions -X-ray photoelectrons -Hidroxiapatita |
Drets:
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Tipus de document:
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Article - Versió publicada Article |
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