Título:
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Probe-specific mixed-model approach to detect copy number differences using multiplex ligation-dependent probe amplification (MLPA)
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Autor/a:
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González, Juan R.; Carrasco Jordan, Josep Lluís; Armengol, Lluís; Villatoro, Sergi; Jover Armengol, Lluís de; Yasui, Yutaka; Estivill, Xavier, 1955-
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Otros autores:
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Universitat de Barcelona |
Abstract:
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Background: MLPA method is a potentially useful semi-quantitative method to detect copy number alterations in targeted regions. In this paper, we propose a method for the normalization procedure based on a non-linear mixed-model, as well as a new approach for determining the statistical significance of altered probes based on linear mixed-model. This method establishes a threshold by using different tolerance intervals that accommodates the specific random error variability observed in each test sample.
Results: Through simulation studies we have shown that our proposed method outperforms two existing methods that are based on simple threshold rules or iterative regression. We have illustrated the method using a controlled MLPA assay in which targeted regions are variable in copy number in individuals suffering from different disorders such as Prader-Willi, DiGeorge or Autism showing the best performace.
Conclusion: Using the proposed mixed-model, we are able to determine thresholds to decide whether a region is altered. These threholds are specific for each individual, incorporating experimental variability, resulting in improved sensitivity and specificity as the examples with real data have revealed. |
Materia(s):
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-Genètica molecular -Biotecnologia -Gene dosage -Molecular probe techniques |
Derechos:
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cc-by, (c) González et al., 2008
http://creativecommons.org/licenses/by/2.0/ |
Tipo de documento:
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Artículo Artículo - Versión publicada |
Editor:
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BioMed Central
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