Oligonucleotide cyclization: The thiol-maleimide reaction revisited

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Title:	Oligonucleotide cyclization: The thiol-maleimide reaction revisited
Author:	Sánchez, Albert; Pedroso Muller, Enrique; Grandas Sagarra, Anna
Other authors:	Universitat de Barcelona
Abstract:	A novel method to synthesize cyclic oligonucleotides (5- to 26-mer) using the thiol-maleimide reaction is described. The target molecules were obtained after subsequent removal of thiol and maleimide protecting groups from 5′-maleimido-3′-thiol-derivatized linear precursors. Retro-Diels-Alder conditions deprotecting the maleimide simultaneously promoted cyclization cleanly and in high yield.
Subject(s):	-Bioquímica -Àcids nucleics -Bacteris -Microbiologia -Ciclització (Química) -Biochemistry -Nucleic acids -Bacteria -Microbiology -Ring formation (Chemistry)
Rights:	(c) Sánchez, Albert et al., 2013
Document type:	Article Article - Accepted version
Published by:	Royal Society of Chemistry
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