Title:
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Sensitive and Rapid UHPLC-MS/MS for the Analysis of Tomato Phenolics in Human Biological Samples
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Author:
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Martínez-Huélamo, M.; Tulipani, S.; Jáuregui Pallarés, Olga; Valderas Martínez, Palmira; Vallverdú i Queralt, Anna; Estruch Riba, Ramon; Torrado, Xavier; Lamuela Raventós, Rosa Ma.
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Other authors:
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Universitat de Barcelona |
Abstract:
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An UHPLC-MS/MS method for the quantification of tomato phenolic metabolites in human fluids was optimized and validated, and then applied in a pilot dietary intervention study with healthy volunteers. A 5-fold gain in speed (3.5 min of total run); 7-fold increase in MS sensitivity and 2-fold greater efficiency (50% peak width reduction) were observed when comparing the proposed method with the reference-quality HPLC-MS/MS system, whose assay performance has been previously documented. The UHPLC-MS/MS method led to an overall improvement in the limits of detection (LOD) and quantification (LOQ) for all the phenolic compounds studied. The recoveries ranged between 68% and 100% in urine and 61% and 100% in plasma. The accuracy; intra- and interday precision; and stability met with the acceptance criteria of the AOAC International norms. Due to the improvements in the analytical method; the total phenolic metabolites detected in plasma and urine in the pilot intervention study were 3 times higher than those detected by HPLC-MS/MS. Comparing with traditional methods; which require longer time of analysis; the methodology described is suitable for the analysis of phenolic compounds in a large number of plasma and urine samples in a reduced time frame. |
Subject(s):
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-Biologia humana -Tomàquets -Metabòlits -Human biology -Tomatoes -Metabolites |
Rights:
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cc-by (c) Martínez-Huélamo, M. et al., 2015
http://creativecommons.org/licenses/by/3.0/es |
Document type:
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Article Article - Published version |
Published by:
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MDPI
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