Title:
|
Escherichia coli and pseudomonas aeruginosa eradication by nano-penicillin G
|
Author:
|
Macedo Fernandes, Margarida Maria; Ivanova, Kristina Dimitrova; Francesko, Antonio; Rivera, Diana; Torrent Burgués, Juan; Mendoza Gómez, Ernesto; Tzanov, Tzanko
|
Other authors:
|
Universitat Politècnica de Catalunya. Departament d'Enginyeria Química; Universitat Politècnica de Catalunya. Departament de Física; Universitat Politècnica de Catalunya. GBMI - Grup de Biotecnologia Molecular i Industrial |
Abstract:
|
The transformation of penicillin G into nano/micro-sized spheres (nanopenicillin) using sonochemical technology was explored as a novel tool for the eradication of Gram-negative bacteria and their biofilms. Known by its effectiveness only against Gram-positive microorganisms, the penicillin G spherization boosted the inhibition of the Gram-negative Pseudomonas aeruginosa 10-fold (from 0.3 to 3.0 log-reduction) and additionally induced 1.2 log-reduction of Escherichia coli growth. The efficient penetration of the spheres within a Langmuir monolayer sustained the theory that nanopenicillin is able to cross the membrane and reach the periplasmic space in Gram-negative bacteria where they inhibit the ß-lactam targets: the transferases that build the bacteria cell wall. Moreover, it considerably suppressed the growth of both bacterial biofilms on a medically relevant polystyrene surface, leaving majority of the adhered cells dead compared to the treatment with the non-processed penicillin G. Importantly, nanopenicillin was found innocuous towards human fibroblasts at the antibacterial-effective concentration |
Abstract:
|
Peer Reviewed |
Subject(s):
|
-Àrees temàtiques de la UPC::Enginyeria química -Bioengineering -Nanoparticles--Therapeutic use -Penicillin G -Sonochemistry -Nano/Microspheres -Nano-bio interactions -Antibacterial -Antibiofilm -Bioenginyeria -Nanopartícules -- Ús terapèutic |
Rights:
|
http://creativecommons.org/licenses/by-nc-nd/3.0/es/ |
Document type:
|
Article - Published version Article |
Share:
|
|