Title:
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Maslinic acid, a natural Triterpene, induces a death receptor-mediated apoptotic mechanism in Caco-2 p53-deficient colon adenocarcinoma cells
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Author:
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Reyes-Zurita, Fernando J.; Rufino Palomares, Eva E.E.; García Salguero, Leticia; Peragón, Juan; Medina, Pedro P.; Parra Puerto, Andrés; Cascante i Serratosa, Marta; Lupiáñez, José A.
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Other authors:
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Universitat de Barcelona |
Abstract:
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Maslinic acid (MA) is a natural triterpene present in high concentrations in the waxy skin of olives. We have previously reported that MA induces apoptotic cell death via the mito- chondrial apoptotic pathway in HT29 colon cancer cells. Here, we show that MA induces apoptosis in Caco-2 colon cancer cells via the extrinsic apoptotic pathway in a dose- dependent manner. MA triggered a series of effects associated with apoptosis, including the cleavage of caspases -8 and -3, and increased the levels of t-Bid within a few hours of its addition to the culture medium. MA had no effect on the expression of the Bax protein, release of cytochrome-c or on the mitochondr ial membrane potential. This suggests that MA triggered the extrinsic apoptotic pathway in this cell type, as opposed to the intrinsic pathway found in the HT29 colon-cancer cell line. Our results suggest that the apoptotic mechanism induced in Caco-2 may be different from that found in HT29 colon-cancer cells, and that in Caco-2 cells MA seems to work independently of p53. Natural antitumoral agents capable of activating both the extrinsic and intrinsic apoptotic pathways could be of great use in treating colon-cancer of whatever origin. |
Subject(s):
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-Olis vegetals -Càncer colorectal -Diferenciació cel·lular -Proteïnes -Vegetable oils -Colorectal cancer -Cell diferentiation -Proteins |
Rights:
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cc-by (c) Reyes-Zurita, Fernando J. et al., 2016
http://creativecommons.org/licenses/by/3.0/es |
Document type:
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Article Article - Published version |
Published by:
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Public Library of Science (PLoS)
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