A multitude of cellular and subcellular processes with pathological relevance depend critically on the mechanical deformability of the cytoplasm. Particle-tracking microrheology measures the viscoelastic properties of the cytoplasm locally and with high spatiotemporal resolution. In this project I worked on different methods to study microrheology on living cells (mouse embryo fibroblasts) and select the one that gives better results to analyze the changes in the cytoplasm under flow conditions.