Título:
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Host age and expression of genes involved in red blood cell
invasion in Plasmodium falciparum field isolates
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Autor/a:
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Valmaseda, Aida; Bassat Orellana, Quique; Aide, Pedro Carlos Paulino; Cisteró, Pau; Jiménez, Alfons; Casellas, Aina; Machevo, Sonia; Aguilar, Ruth; Sigaúque, Betuel; Chauhan, Virander Singh; Langer, Christine; Beeson, James G.; Chitnis, Chetan E.; Alonso, Pedro; Gaur, Deepak; Mayor Aparicio, Alfredo Gabriel
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Abstract:
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Plasmodium falciparum proteins involved in erythrocyte invasion
are main targets of acquired immunity and important vaccine
candidates. We hypothesized that anti-parasite immunity acquired
upon exposure would limit invasion-related gene (IRG) expression
and affect the clinical impact of the infection. 11 IRG
transcript levels were measured in P. falciparum isolates by
RT-PCR, and IgG/IgM against invasion ligands by Luminex(R), in
50 Mozambican adults, 25 children with severe malaria (SM) and
25 with uncomplicated malaria (UM). IRG expression differences
among groups and associations between IRG expression and
clinical/immunologic parameters were assessed. IRG expression
diversity was higher in parasites infecting children than adults
(p = 0.022). eba140 and ptramp expression decreased with age (p
= 0.003 and 0.007, respectively) whereas p41 expression
increased (p = 0.022). pfrh5 reduction in expression was abrupt
early in life. Parasite density decreased with increasing pfrh5
expression (p < 0.001) and, only in children, parasite
density increased with p41 expression (p = 0.007), and decreased
with eba175 (p = 0.013). Antibody responses and IRG expression
were not associated. In conclusion, IRG expression is associated
with age and parasite density, but not with specific antibody
responses in the acute phase of infection. Our results confirm
the importance of multi-antigen vaccines development to avoid
parasite immune escape when tested in malaria-exposed
individuals. |
Materia(s):
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-Plasmodium falciparum -Hematies -Plasmodium falciparum -Erythrocytes |
Derechos:
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cc by (c) Valmaseda, Aida et al., 2017
http://creativecommons.org/licenses/by/3.0/es/ |
Tipo de documento:
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Artículo Artículo - Versión publicada |
Editor:
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Nature Publishing
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Compartir:
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