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Título:	Improving virus production through quasispecies genomic selection and molecular breedings
Autor/a:	Pérez Rodríguez, Francisco Javier, 1964-; D'Andrea, Lucia; Castellarnau Serra, Montserrat de; Costafreda Salvany, M. Isabel; Guix Arnau, Susana; Ribes Mora, Enric; Quer, Josep; Gregori Font, Josep; Bosch, Albert; Pintó Solé, Rosa María
Otros autores:	Universitat de Barcelona
Abstract:	Virus production still is a challenging issue in antigen manufacture, particularly with slow-growing viruses. Deep-sequencing of genomic regions indicative of efficient replication may be used to identify high-fitness minority individuals suppressed by the ensemble of mutants in a virus quasispecies. Molecular breeding of quasispecies containing colonizer individuals, under regimes allowing more than one replicative cycle, is a strategy to select the fittest competitors among the colonizers. A slow-growing cell culture-adapted hepatitis A virus strain was employed as a model for this strategy. Using genomic selection in two regions predictive of efficient translation, the internal ribosome entry site and the VP1-coding region, high-fitness minority colonizer individuals were identified in a population adapted to conditions of artificially-induced cellular transcription shut-off. Molecular breeding of this population with a second one, also adapted to transcription shut-off and showing an overall colonizer phenotype, allowed the selection of a fast-growing population of great biotechnological potential.
Materia(s):	-Virologia -Espècies (Biologia) -Virology -Species
Derechos:	cc-by (c) Pérez et al., 2016 http://creativecommons.org/licenses/by/3.0/es
Tipo de documento:	Artículo Artículo - Versión publicada
Editor:	Nature Publishing Group
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