Title:
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Linking biofilm spatial structure to real-time microscopic oxygen decay imaging
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Author:
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Rubol, Simonetta; Freixa, Anna; Sánchez Vila, Francisco Javier; Romani Cornet, Anna Maria
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Other authors:
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Universitat Politècnica de Catalunya. Departament d'Enginyeria Civil i Ambiental; Universitat Politècnica de Catalunya. GHS - Grup d'Hidrologia Subterrània |
Abstract:
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This is an Accepted Manuscript of an article published by Taylor & Francis Group in Biofouling on 2018, available online at: http://www.tandfonline.com/10.1080/08927014.2017.1423474 |
Abstract:
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Two non-destructive techniques, confocal laser scanning microscopy (CLSM) and planar optode (VisiSens imaging), were combined to relate the fine-scale spatial structure of biofilm components to real-time images of oxygen decay in aquatic biofilms. Both techniques were applied to biofilms grown for seven days at contrasting light and temperature (10/20°C) conditions. The geo-statistical analyses of CLSM images indicated that biofilm structures consisted of small (~100 µm) and middle sized (~101 µm) irregular aggregates. Cyanobacteria and EPS (extracellular polymeric substances) showed larger aggregate sizes in dark grown biofilms while, for algae, aggregates were larger in light-20°C conditions. Light-20°C biofilms were most dense while 10°C biofilms showed a sparser structure and lower respiration rates. There was a positive relationship between the number of pixels occupied and the oxygen decay rate. The combination of optodes and CLMS, taking advantage of geo-statistics, is a promising way to relate biofilm architecture and metabolism at the micrometric scale. |
Abstract:
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Peer Reviewed |
Subject(s):
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-Àrees temàtiques de la UPC::Enginyeria civil::Enginyeria hidràulica, marítima i sanitària::Enginyeria sanitària -Àrees temàtiques de la UPC::Enginyeria química::Química del medi ambient::Geoquímica -Oxygen -Geochemistry -Confocal laser scanning microscopy -real-time images of oxygen concentration -biofilm growth -planar optodes -biofilm respiration -geostatistics -Oxigen -Geoquímica ambiental |
Rights:
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Document type:
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Article - Submitted version Article |
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