To access the full text documents, please follow this link: http://hdl.handle.net/10230/34215

Title:	Non-linear formation of EtG and FAEEs after controlled administration of low to moderate doses of ethanol
Author:	Pérez Mañá, Clara; Farré Albaladejo, Magí; Pastor, Antonio; Fonseca Casals, Francina, 1972-; Torrens, Marta; Menoyo Colomer, Esther; Pujadas Bastardes, Mitona; Frias, Silvia; Langohr, Klaus; Torre Fornell, Rafael de la
Abstract:	AIMS: Ethyl glucuronide (EtG) and fatty acid ethyl esters (FAEEs) are non-oxidative metabolites of alcohol that can be detected in conventional and non-conventional biological matrices for longer periods than alcohol. The aim was to describe the time courses of both biomarkers after ingestion of acute low-moderate doses of ethanol. METHODS: The study design was double-blind, randomized, crossover and controlled with placebo. Participants were distributed in three different cohorts: (a) Cohort-1: two doses of 18 and 30 g of ethanol and placebo were administered to 12 subjects; (b) Cohort-2: two doses of 6 and 12 g of ethanol and placebo were administered to six subjects and (c) Cohort-3: two doses of 24 and 42 g of ethanol and placebo were administered to six subjects. Each participant received two doses of ethanol and placebo. Plasma concentrations (0-6 h) of ethanol and specific FAEEs (palmitic, stearic, linoleic and oleic acid ethyl esters) and urinary concentrations of EtG (0-24 h) were measured. RESULTS: A dose-dependent increase in blood ethanol concentrations was observed. EtG excretion and FAEEs plasmatic concentrations showed a disproportionate increase with the ethanol dose suggesting non-linearity. Area under the curve (AUC0-6h) of ethanol concentrations showed a linear trend with non-oxidative metabolites' concentrations. CONCLUSION: The formation rate of ethanol non-oxidative biomarkers does not follow a linear trend, explained mainly by a disproportionate increase in AUC0-6h of ethanol concentrations in relation to dose. This observation should be taken into account when interpreting results in biological matrices in clinical and forensic settings. SHORT SUMMARY: A double-blind, randomized, crossover and controlled study was conducted administering ethanol (6-42 g). Ethyl glucuronide (EtG) excretion and fatty acid ethyl esters (FAEEs) plasmatic concentrations showed a disproportionate increase with the ethanol dose suggesting non-linearity. This observation should be taken into account when interpreting biomarker concentrations in clinical settings.
Subject(s):	-Marcadors bioquímics -Alcohol -- Efectes fisiològics -Ethanol -Esters
Rights:	info:eu-repo/semantics/embargoedAccess © Oxford University Press. This is a pre-copy-editing, author-produced PDF of an article accepted for publication in Alcohol and alcoholism following peer review. The definitive publisher-authenticated version Pérez-Mañá C, Farré M, Pastor A, Fonseca F, Torrens M, Menoyo E. et al. Non-Linear Formation of EtG and FAEEs after Controlled Administration of Low to Moderate Doses of Ethanol. Alcohol Alcohol. 2017 Sep 1;52(5):587-594 is available online at: http://dx.doi.org/10.1093/alcalc/agx033
Document type:	Article Article - Accepted version
Published by:	Oxford University Press
Share:

Show full item record