Pancreatic Ductal Adenocarcinoma (PDAC) therapies show limited success./nIrreversible electroporation (IRE) is an innovative loco-regional therapy in which highvoltage pulses are applied to induce plasma membrane defects leading to cellular death. In the present study we evaluated the feasibility of IRE against PDAC. IRE/ntreatment exhibited significant antitumor effects and prolonged survival in mice with orthotopic xenografts. Extensive tumor necrosis, reduced tumor cell proliferation and disruption of microvessels were observed at different days post-IRE. Animals had transient increases in transaminases, amylase and lipase enzymes that normalized at/n24h post-IRE. These results suggest that IRE could be an effective treatment for locally advanced pancreatic tumors.

This work was supported by the Spanish/nMinistry of Science and Innovation (MICINN), BIO2008-04692-C03-02/01 and received/npartial support from the Generalitat de Catalunya SGR091527. Anabel José was a/nrecipient of a FPU fellowship from the MICINN. Fillat’s group is also partially funded by/nCIBERER and by IIS10/00014 from Instituto de Salud Carlos III. Ivorra’s research is/ncurrently supported by a Ramón y Cajal fellowship from the Spanish Ministry for/nScience and Innovation and a Marie Curie IRG grant from the European Commission.