Títol:
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Influence of absorption and scattering on the quantification of fluorescence diffuse optical tomography using normalized data
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Autor/a:
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Abascal, Juan F.P.J.; Aguirre, Juan; Chamorro Servent, Judit; Schweiger, Martin; Arridge, Simon; Ripoll, Jorge; Vaquero, Juan J.
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Abstract:
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Reconstruction algorithms for imaging fluorescence in near infrared ranges usually normalize fluorescence
light with respect to excitation light. Using this approach, we investigated the influence of absorption
and scattering heterogeneities on quantification accuracy when assuming a homogeneous model and explored
possible reconstruction improvements by using a heterogeneous model. To do so, we created several computer-
simulated phantoms: a homogeneous slab phantom (P1), slab phantoms including a region with a two- to
six-fold increase in scattering (P2) and in absorption (P3), and an atlas-based mouse phantom that modeled different
liver and lung scattering (P4). For P1, reconstruction with the wrong optical properties yielded quantification errors
that increased almost linearly with the scattering coefficient while they were mostly negligible regarding the absorption
coefficient. This observation agreed with the theoretical results. Taking the quantification of a homogeneous
phantom as a reference, relative quantification errors obtained when wrongly assuming homogeneous media were
in the range þ41 to þ94% (P2), 0.1 to −7% (P3), and −39 to þ44% (P4). Using a heterogeneous model, the overall
error ranged from −7 to 7%. In conclusion, this work demonstrates that assuming homogeneous media leads to
noticeable quantification errors that can be improved by adopting heterogeneous models. |
Abstract:
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This study was supported by Ministerio de Ciencia e Innovación
(FPI program, TEC 2008-06715, and CENIT AMIT CEN-
20101014), Comunidad de Madrid and European Regional
Development Fund ARTEMIS S2009/DPI-1802, and EU-FP7
project FMTXCT-201792. |
Matèries:
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-Tomography -Diffusion -Fluorescence -Scattering |
Drets:
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© (2012) Society of Photo-Optical Instrumentation Engineers (SPIE). One print or electronic copy may be made for personal use only. Systematic reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited. Abascal JFPJ, Aguirre J, Chamorro Servent J, Schweiger M, Arridge S, Ripoll J, Vaquero JJ. Influence of absorption and scattering on the quantification of fluorescence diffuse optical tomography using normalized data. Journal of biomedical optics. Journal of Biomedical Optics. 2012;17(3):036013. http://dx.doi.org/10.1117/1.JBO.17.3.036013 |
Tipus de document:
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Article Article - Versió publicada |
Publicat per:
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Society of Photo Optical Instrumentation Engineers (SPIE)
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