dc.contributor.author |
Abadias i Sero, Mª Isabel |
dc.contributor.author |
Altisent Rosell, Rosa |
dc.contributor.author |
Usall i Rodié, Josep |
dc.contributor.author |
Torres Sanchis, Rosario |
dc.contributor.author |
Sousa Oliveira, Márcia Patrícia de |
dc.contributor.author |
Viñas Almenar, Inmaculada |
dc.date |
2017-05-19T13:33:41Z |
dc.date |
2014 |
dc.date |
2017-05-19T13:33:42Z |
dc.date |
10000-01-01 |
dc.identifier |
0925-5214 |
dc.identifier |
http://hdl.handle.net/10459.1/59659 |
dc.identifier |
https://doi.org/10.1016/j.postharvbio.2014.05.010 |
dc.identifier.uri |
http://hdl.handle.net/10459.1/59659 |
dc.description |
The use of biopreservation is a promising technique to ensure microbial safety of fresh-cut produce. The objective of this work was to test the effectiveness of the strain CPA-7 of Pseudomonas graminis against a cocktail of Salmonella spp. and Listeria monocytogenes on fresh-cut melon, and evaluate its effect on its quality during shelf-life when tested in conditions simulating commercial application. Fresh-cut melon was artificially inoculated with Salmonella spp. and L. monocytogenes and with or without the biopreservative strain at different concentrations and stored at 20, 10 and 5 °C. Moreover, the effect of the strain was tested in conditions simulating commercial application. Fresh-cut melon was packaged using passive modified atmosphere (MAP) and AIR conditions and stored at 5 and 10 °C. Quality of fresh-cut melon was evaluated in CPA-7 treated and untreated samples. At laboratory scale trials, P. graminis reduced Salmonella and L. monocytogenes growth on fresh-cut melon stored at 5, 10 and 20 °C. Effectiveness depended on their concentration and on storage temperature. At low pathogen concentration and 20 °C, L. monocytogenes growth was reduced between 2.1 and 5.3 log cfu g−1 units after 2 days of storage and Salmonella growth between 2.0 and 7.3 log cfu g−1 depending on CPA-7 dose. At 10 °C, similar reductions of pathogens were observed after 5 days of storage. In studies simulating commercial conditions, packaging atmosphere and temperature influenced P. graminis effectiveness, with better results in samples packaged under AIR conditions and 10 °C. Reduction of pathogen growth was <1-log unit in fresh-cut melon stored in MAP whilst it was >4-log units in AIR. Soluble solids content, titratable acidity, pH and firmness of fresh-cut melon were not significantly different in CPA-7 treated and untreated (control) melon. In general, lightness, chroma and hue values of fresh-cut melon stored in AIR decreased faster in CPA-7 samples than on control ones. At 5 °C, CPA-7 treated melon was visually scored lower than untreated melon. P. graminis has demonstrated promising results at 10 °C, which is a temperature more compromised for safety. Nevertheless more detailed studies on the modified atmosphere are required because AIR packaging is not recommended due to the rapid loss of quality. |
dc.description |
Authors are grateful to the Spanish Government for research projects AGL-2009-08506 and AGL-2010-19385. |
dc.format |
application/pdf |
dc.language |
eng |
dc.publisher |
Elsevier B.V. |
dc.relation |
MICINN/PN2008-2011/AGL2009-08506 |
dc.relation |
MICINN/PN2008-2011/AGL-2010-19385 |
dc.relation |
Reproducció del document publicat a: https://doi.org/10.1016/j.postharvbio.2014.05.010 |
dc.relation |
Postharvest Biology and Technology, 2014, vol. 96, p. 69 -77 |
dc.rights |
(c) Elsevier, 2014 |
dc.rights |
info:eu-repo/semantics/restrictedAccess |
dc.subject |
Foodborne pathogens |
dc.subject |
Antagonist |
dc.subject |
Microbial safety |
dc.subject |
Salmonella |
dc.title |
Biopreservation of fresh-cut melon using the strain Pseudomonas graminis CPA-7 |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/publishedVersion |