dc.contributor.author |
Soto Muñoz, Lourdes |
dc.contributor.author |
Torres Sanchis, Rosario |
dc.contributor.author |
Usall i Rodié, Josep |
dc.contributor.author |
Viñas Almenar, Inmaculada |
dc.contributor.author |
Solsona Aixalà, Cristina |
dc.contributor.author |
Teixidó i Espasa, Neus |
dc.date |
2017-05-24T09:01:52Z |
dc.date |
2015 |
dc.date |
2017-05-24T09:01:55Z |
dc.date |
10000-01-01 |
dc.identifier |
0168-1605 |
dc.identifier |
http://hdl.handle.net/10459.1/59686 |
dc.identifier |
https://doi.org/10.1016/j.ijfoodmicro.2015.06.013 |
dc.identifier.uri |
http://hdl.handle.net/10459.1/59686 |
dc.description |
Pantoea agglomerans strain CPA-2 is an effective biocontrol agent (BCA) against the major postharvest pathogens present on pome and citrus fruits. Dehydration, such as freeze-drying, spray-drying and fluidized bed drying is one of the best ways to formulate BCAs. In this work, the survival of CPA-2 cells after formulation was determined by dilution plating and molecular methods as qPCR alone and combined with a sample pretreatment with a propidium monoazide dye (PMA-qPCR) and they were used to calculate treatment concentrations in efficacy trials on postharvest oranges. Furthermore, no significant differences in CPA-2 survival were observed as determined by dilution plating and PMA-qPCR after both the freeze drying and fluidized bed drying processes; however, an interesting significant difference was observed in the spray dried product comparing all quantitative methods. A difference of 0.48 and 2.17 log10 CFU or cells g/dw was observed among PMA-qPCR with qPCR and dilution plating, respectively. According to our study, dilution plating was shown to be an unreliable tool for monitoring the survival of CPA-2 after spray drying. In contrast, the combination of PMA and qPCR enabled a quick and unequivocal methodology to enumerate viable and VBNC CPA-2 cells under stress-dried conditions. Efficacy trials showed that, after 3 days, spray drying formulation rehydrated with 10% non-fat skimmed milk (NFSM) was as effective as fresh cells to control Penicillium digitatum in oranges. |
dc.description |
The authors would like to thank Svetlana Dashevskaya for her excellent technical assistance. The authors are grateful to the Spanish Government for the financial support by the national project RTA2009-0053-00-00 (Plan Nacional de I + D + I, Ministerio de Ciencia e Innovación, Spain) and to the National Council of Science and Technology of México (CONACyT) for L. Soto PhD grant 198363. |
dc.format |
application/pdf |
dc.language |
eng |
dc.publisher |
Elsevier B.V. |
dc.relation |
MICINN/PN2008-2011/RTA2009-0053-00-00 |
dc.relation |
Reproducció del document publicat a: https://doi.org/10.1016/j.ijfoodmicro.2015.06.013 |
dc.relation |
International Journal of Food Microbiology, 2015, vol. 210, p. 79-83 |
dc.rights |
(c) Elsevier, 2015 |
dc.rights |
info:eu-repo/semantics/restrictedAccess |
dc.subject |
Bed fluidized-drying |
dc.subject |
Dilution plating |
dc.subject |
Freeze-drying |
dc.subject |
PMA |
dc.title |
DNA-based methodologies for the quantification of live and dead cells in formulated biocontrol products based on Pantoea agglomerans CPA-2 |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/publishedVersion |