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Título:
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Yeast as a model system to study metabolic impact of selenium compounds
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Autor/a:
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Herrero Perpiñán, Enrique; Wellinger, Ralf Erik
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Notas:
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Inorganic Se forms such as selenate or selenite (the two more
abundant forms in nature) can be toxic in Saccharomyces cerevisiae cells,
which constitute an adequate model to study such toxicity at the molecular
level and the functions participating in protection against Se compounds.
Those Se forms enter the yeast cell through other oxyanion transporters.
Once inside the cell, inorganic Se forms may be converted into selenide
through a reductive pathway that in physiological conditions involves reduced
glutathione with its consequent oxidation into diglutathione and alteration of
the cellular redox buffering capacity. Selenide can subsequently be converted
by molecular oxygen into elemental Se, with production of superoxide anions
and other reactive oxygen species. Overall, these events result in DNA damage
and dose-dependent reversible or irreversible protein oxidation, although
additional oxidation of other cellular macromolecules cannot be discarded.
Stress-adaptation pathways are essential for efficient Se detoxification, while
activation of DNA damage checkpoint and repair pathways protects against
Se-mediated genotoxicity. We propose that yeast may be used to improve our
knowledge on the impact of Se on metal homeostasis, the identification of Setargets
at the DNA and protein levels, and to gain more insights into the
mechanism of Se-mediated apoptosis.
This work was supported by grants from the Universitat de Lleida to E.H. and from the Junta de Andalucía [P11-CTS- 7962]/European Union (FEDER) to R.E.W. |
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Materia(s):
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-Selenium
-Yeast
-DNA damage
-Oxidative stress |
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Derechos:
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cc-by (c) Herrero et al., 2015
http://creativecommons.org/licenses/by/4.0/
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Tipo de documento:
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article
publishedVersion |
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Editor:
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Shared Science Publishers
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Compartir:
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