dc.contributor.author |
Gotor Vila, Amparo María |
dc.contributor.author |
Teixidó i Espasa, Neus |
dc.contributor.author |
Usall i Rodié, Josep |
dc.contributor.author |
Dashevskaya, Svetlana |
dc.contributor.author |
Torres Sanchis, Rosario |
dc.date |
2018-04-17T10:33:19Z |
dc.date |
2018-04-17T10:33:19Z |
dc.date |
2016 |
dc.identifier |
0003-4746 |
dc.identifier |
http://hdl.handle.net/10459.1/63108 |
dc.identifier |
https://doi.org/10.1111/aab.12298 |
dc.identifier.uri |
http://hdl.handle.net/10459.1/63108 |
dc.description |
In this work, reliable tools were developed to detect and identify the biocontrol
strain CPA-8 using DNA amplification techniques. As a first approach, the
RAPD (random amplified polymorphic DNA) technique was applied to a
collection of 77 related Bacillus species. Among the primers tested, the primer
pair OPG1/OPG6 amplified a 668 bp specific product to the strain CPA-8 that
was sequenced and used to design SCAR (sequence-characterised amplified
regions) primer pairs. The SCAR-4 marker amplified a semi-specific fragment
of 665 bp not only for the strain CPA-8 but also for other 12 strains whose
morphology was completely different from CPA-8. Another approach was
developed to obtain a strain-specific genomic marker related to ecological
adaptations of Bacillus amyloliquefaciens species. The primer pair F2/R2 obtained
from RBAM 007760, a gene involved in surface adhesion, amplified a 265 bp
fragment unique for strain CPA-8. Our results revealed that these two molecular
markers, SCAR-4 and RBAM 007760 F2/R2 provide suitable monitoring
tools to specifically identify the biocontrol CPA-8 when applied against brown
rot caused by Monilinia spp. in stone fruit. Moreover, our findings demonstrate
that the strain CPA-8 is affiliated with B. amyloliquefaciens species that was
formerly designated as Bacillus subtilis. |
dc.description |
This research was supported by the European project BIOCOMES FP7-612713 and by the Catalan government (Generalitat de Catalunya) for the PhD grant 2015-FI-B100166 (Amparo M. Gotor). No conflict of interest declared. |
dc.language |
eng |
dc.publisher |
Wiley |
dc.publisher |
Association of Applied Biologists |
dc.relation |
Versió postprint del document publicat a https://doi.org/10.1111/aab.12298 |
dc.relation |
Annals of Applied Biology, 2016, vol. 169, núm. 2, p. 248–256 |
dc.relation |
info:eu-repo/grantAgreement/EC/FP7/612713 |
dc.rights |
© Association of Applied Biologists, 2016 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
Bacillus spp |
dc.subject |
Biological control |
dc.subject |
Molecular marker |
dc.subject |
Monitoring |
dc.subject |
SCAR |
dc.title |
Development of a SCAR marker and a strain-specific genomic marker for the detection of the biocontrol agent strain CPA-8 Bacillus amyloliquefaciens (formerly B. subtilis) |
dc.type |
article |
dc.type |
acceptedVersion |