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Título:	Multiplex antibody analysis of IgM, IgA and IgG to SARS-CoV-2 in saliva and serum from infected children and their close contacts
Autor/a:	Dobaño, Carlota; Alonso, Selena; Vidal, Marta; Jiménez, Alfons; Rubio, Rocío; Santano, Rebeca; Barrios, Diana; Pons Tomas, Gemma; Melé Casas, María; Hernández García, María; Girona-Alarcón, Mònica; Puyol, Laura; Baro, Barbara; Millat-Martínez, Pere; Ajanovic, Sara; Balanza, Núria; Arias, Sara; Rodrigo Melero, Natalia; Carolis, Carlo; García-Miquel, Aleix; Bonet-Carné, Elisenda; Claverol, Joana; Cubells, Marta; Fortuny, Claudia; Fumadó, Victoria; Codina, Anna; Bassat, Quique; Muñoz-Almagro, Carmen; Fernández de Sevilla, Mariona; Gratacós, Eduard; Izquierdo, Luis; García-García, Juan José; Aguilar, Ruth; Jordan, Iolanda; Moncunill, Gemma
Notas:	COVID-19 affects children to a lesser extent than adults but they can still get infected and transmit SARS-CoV-2 to their contacts. Field deployable non-invasive sensitive diagnostic techniques are needed to evaluate the infectivity dynamics of SARS-CoV-2 in pediatric populations and guide public health interventions, particularly if this population is not fully vaccinated. We evaluated the utility of high-throughput Luminex assays to quantify saliva IgM, IgA and IgG antibodies against five SARS-CoV-2 spike (S) and nucleocapsid (N) antigens in a contacts and infectivity longitudinal study in 122 individuals (52 children and 70 adults). We compared saliva versus serum/plasma samples in infected children and adults diagnosed by weekly RT-PCR over 35 days (n=62), and those who consistently tested negative over the same follow up period (n=60), in the Summer of 2020 in Barcelona, Spain. Saliva antibody levels in SARS-CoV-2 RT-PCR positive individuals were significantly higher than in negative individuals and correlated with those measured in sera/plasmas. Asymptomatic infected individuals had higher levels of anti-S IgG than symptomatic individuals, suggesting a protective anti-disease role for antibodies. Higher anti-S IgG and IgM levels in serum/plasma and saliva, respectively, in infected children compared to infected adults could also be related to stronger clinical immunity in them. Among infected children, males had higher levels of saliva IgG to N and RBD than females. Despite overall correlation, individual clustering analysis suggested that responses that may not be detected in blood could be patent in saliva, and vice versa.
Materia(s):	-COVID-19 -Saliva -Complex d'anticossos -Antigen -Nens -Plasma -Sèrum -Vigilància -COVID-19 -Saliva -Complejo anticuerpo-antígeno -Niños -Plasma -Suero -Vigilancia -COVID-19 -Saliva -Antibody complex -Antigen -Children -Plasma -Serum -Surveillance -61 -616.9
Derechos:	© 2022 Dobaño, Alonso, Vidal, Jiménez, Rubio, Santano, Barrios, Pons Tomas, Melé Casas, Hernández García, Girona-Alarcón, Puyol, Baro, Millat-Martínez, Ajanovic, Balanza, Arias, Rodrigo Melero, Carolis, García-Miquel, Bonet-Carné, Claverol, Cubells, Fortuny, Fumadó, Codina, Bassat, Muñoz-Almagro, Fernández de Sevilla, Gratacós, Izquierdo, García-García, Aguilar, Jordan and Moncunill. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. https://creativecommons.org/licenses/by/4.0/
Tipo de documento:	Artículo Artículo - Versión publicada
Editor:	Frontiers Media
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