dc.contributor |
IRTA. Recerca i Tecnologia Agroalimentàries |
dc.contributor.author |
Torres, E. |
dc.contributor.author |
Laviña Gomila, Amparo |
dc.contributor.author |
Sabaté, J. |
dc.contributor.author |
Bech, J. |
dc.contributor.author |
Batlle i Durany, Assumpció |
dc.date.accessioned |
2011-12-14T14:20:50Z |
dc.date.available |
2011-12-14T14:20:50Z |
dc.date.created |
2010 |
dc.date.issued |
2010 |
dc.identifier.citation |
Torres E. et al (2010) " Evaluation of susceptibility of pear and plum varieties and rootstocks to Ca. P. pyri and Ca. P. prunorum using Real-Time PCR." 21st International Conference on Virus and other Graft Transmissible Diseases of Fruit Crops : July 5 - 10, 2009, Neustadt, Germany.ISBN 978-3-930037-67-4. Julius-Kühn-Archiv, 427 p.395-398 |
dc.identifier.uri |
http://hdl.handle.net/2072/179101 |
dc.format.extent |
4 p. |
dc.language.iso |
eng |
dc.publisher |
Julius-Kühn-Archiv |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.rights |
L'accés als continguts d'aquest document queda condicionat a l'acceptació de les condicions d'ús establertes per la següent llicència Creative Commons: http://creativecommons.org/licenses/by-nc-sa/3.0/es/ |
dc.source |
RECERCAT (Dipòsit de la Recerca de Catalunya) |
dc.subject.other |
Fitoplasma |
dc.title |
Evaluation of susceptibility of pear and plum varieties and rootstocks to Ca. P. pyri and Ca. P. prunorum using Real-Time PCR |
dc.type |
info:eu-repo/semantics/article |
dc.subject.udc |
631 - Agricultura. Agronomia. Maquinària agrícola. Sòls. Edafologia agrícola |
dc.embargo.terms |
cap |
dc.description.abstract |
Real-time PCR was used to quantify phytoplasma concentration in fifty inoculated trees from five Prunus rootstocks
and in forty-eight symptomatic pear and Japanese plum trees from orchards. Seasonal fluctuation of Ca. P. prunorum in
different Prunus rootstocks, over three years, showed that the highest percentage detected by nested-PCR was in the
‘Garnem’ rootstock on nearly all sampling dates. Intra-varietal differences were also observed. Phytoplasma titer could
be estimated by real time PCR in some trees of the rootstocks ‘Garnem’, ‘Barrier’, ‘GF-677’ and ‘Marianna’, and
ranged from 4.7x105 to 3.18x109 phytoplasmas per gram of tissue. Quantification by real-time PCR was not possible in
the ‘Cadaman’ trees analyzed, probably due to a lower phytoplasma titer in this variety. Samples from infected trees
from commercial plots had different phytoplasma concentration and detection percentage depending on the variety,
both being lower in ‘Fortune’ and ‘606’ Japanese plum and in ‘Blanquilla’ pear trees. |