Mechanisms involved in down-regulation of intestinal IgA in rats by high cocoa intake

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RECERCAT Home > Universitat de Barcelona > Bioquímica i Fisiologia > Articles publicats en revistes (Bioquímica i Fisiologia) > View document

To access the full text documents, please follow this link: http://hdl.handle.net/2445/28906

dc.contributor	Universitat de Barcelona
dc.contributor.author	Pérez Berezo, Teresa
dc.contributor.author	Franch i Masferrer, Àngels
dc.contributor.author	Castellote i Bargalló, M. Cristina
dc.contributor.author	Castell, Margarida
dc.contributor.author	Pérez-Cano, Francisco J.
dc.date	2012-07-27T10:19:19Z
dc.date	2012-07-27T10:19:19Z
dc.date	2012-07
dc.date	2012-07-27T10:19:19Z
dc.identifier.citation	0955-2863
dc.identifier.citation	584955
dc.identifier.uri	http://hdl.handle.net/2445/28906
dc.description.abstract	Previous studies have shown that rat intestinal immunoglobulin A (IgA) concentration and lymphocyte composition of the intestinal immune system were influenced by a highly enriched cocoa diet. The aim of this study was to dissect the mechanisms by which a long-term high cocoa intake was capable of modifying gut secretory IgA in Wistar rats. After 7 weeks of nutritional intervention, Peyer's patches, mesenteric lymph nodes and the small intestine were excised for gene expression assessment of IgA, transforming growth factor ß, C-C chemokine receptor-9 (CCR9), interleukin (IL)-6, CD40, retinoic acid receptors (RAR¿ and RARß), C-C chemokine ligand (CCL)-25 and CCL28 chemokines, polymeric immunoglobulin receptor and toll-like receptors (TLR) expression by real-time polymerase chain reaction. As in previous studies, secretory IgA concentration decreased in intestinal wash and fecal samples after cocoa intake. Results from the gene expression showed that cocoa intake reduced IgA and IL¿6 in Peyer's patches and mesenteric lymph nodes, whereas in small intestine, cocoa decreased IgA, CCR9, CCL28, RAR¿ and RARß. Moreover, cocoa-fed animals presented an altered TLR expression pattern in the three compartments studied. In conclusion, a high-cocoa diet down-regulated cytokines such as IL-6, which is required for the activation of B cells to become IgA-secreting cells, chemokines and chemokine receptors, such as CCL28 and CCR9 together with RAR¿ and RARß, which are involved in the gut homing of IgA-secreting cells. Moreover, cocoa modified the cross-talk between microbiota and intestinal cells as was detected by an altered TLR pattern. These overall effects in the intestine may explain the intestinal IgA down-regulatory effect after the consumption of a long-term cocoa-enriched diet.
dc.format	7 p.
dc.format	application/pdf
dc.language.iso	eng
dc.publisher	Elsevier B.V.
dc.relation	Versió postprint del document publicat a: http://dx.doi.org/10.1016/j.jnutbio.2011.04.008
dc.relation	Journal of Nutritional Biochemistry, 2012, vol. 23, num. 7, p. 838-844
dc.relation	http://dx.doi.org/10.1016/j.jnutbio.2011.04.008
dc.rights	(c) Elsevier B.V., 2012
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	Flavonoides
dc.subject	Sistema immunològic
dc.subject	Intestins
dc.subject	Cacau
dc.subject	Flavonoids
dc.subject	Immune system
dc.subject	Intestines
dc.subject	Cocoa
dc.title	Mechanisms involved in down-regulation of intestinal IgA in rats by high cocoa intake
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/acceptedVersion

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