dc.contributor |
Universitat de Barcelona |
dc.contributor.author |
Gao, Su |
dc.contributor.author |
Serra i Cucurull, Dolors |
dc.contributor.author |
Keung, Wendy |
dc.contributor.author |
García Hegardt, Fausto |
dc.contributor.author |
Lopaschuk, Gary D. |
dc.date |
2014-05-19T13:16:55Z |
dc.date |
2013-08-01 |
dc.date |
2014-05-16T10:51:45Z |
dc.identifier.citation |
0193-1849 |
dc.identifier.citation |
637821 |
dc.identifier.uri |
http://hdl.handle.net/2445/54185 |
dc.format |
31 p. |
dc.format |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
American Physiological Society |
dc.relation |
Versió postprint del document publicat a: http://dx.doi.org/10.1152/ajpendo.00168.2013 |
dc.relation |
American Journal of Physiology-Endocrinology and Metabolism, 2013, vol. 305, num. 3, p. E336-E347 |
dc.relation |
http://dx.doi.org/10.1152/ajpendo.00168.2013 |
dc.rights |
(c) American Physiological Society, 2013 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.subject |
Nutrició |
dc.subject |
Fisiologia |
dc.subject |
Hipotàlem |
dc.subject |
Carnitina palmitoïl-transferasa 1 |
dc.subject |
Nutrition |
dc.subject |
Physiology |
dc.subject |
Hypothalamus |
dc.subject |
Carnitine palmitoyltransferase I |
dc.title |
Important role of ventromedial hypothalamic carnitine palmitoyltransferase-1a in the control of food intake |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/acceptedVersion |
dc.description.abstract |
Carnitine palmitoyltransferase-1 (CPT-1) liver isoform or CPT-1a is implicated in CNS control of food intake. However, the exact brain nucleus site(s) in mediating this action of CPT-1a has not been identified. In this report, we assess the role of CPT-1a in hypothalamic ventromedial nucleus (VMN). We stereotaxically injected an adenoviral vector containing CPT-1a coding sequence into the VMN of rats to induce overexpression and activation of CPT-1a. The VMN-selective activation of CPT-1a induced orexigenic effect, suggesting CPT-1a in the VMN is involved in the central control of feeding. Intracerebroventricular administration of etomoxir, a CPT-1 inhibitor, decreases food intake. Importantly, in the animals with VMN-overexpression of a CPT-1a mutant that antagonizes the CPT-1 inhibition by etomoxir, the anorectic response to etomoxir was attenuated. This suggests that VMN is involved in mediating the anorectic effect of central inhibition of CPT-1a. In contrast, Arc overexpression of the mutant did not alter etomoxir-induced inhibition of food intake, suggesting that Arc CPT-1a does not play significant roles in this anorectic action. Furthermore, in the VMN, CPT-1a appears to act downstream of hypothalamic malonyl-CoA action of feeding. Finally, we show that in the VMN, CPT-1 activity altered in concert with fasting and refeeding states, supporting a physiological role of CPT-1a in mediating the control of feeding. Taking together, CPT-1a in the hypothalamic VMN appears to play an important role in the central control of food intake. VMN-selective modulation of CPT-1a activity may therefore be a promising strategy in controlling food intake and maintaining normal body weight. |