Development of a 16S rRNA PCR-RFLP Assay for Bartonella identification: Applicability in the identification of species involved in human infections

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dc.contributor	Universitat Politècnica de Catalunya. Departament d'Enginyeria Química
dc.contributor	Universitat Politècnica de Catalunya. PSEP - Polimers Sintètics: Estructura i Propietats. Polimers Biodegradables.
dc.contributor.author	Valle Mendoza, Luis Javier del
dc.contributor.author	Jaramillo, Michael L.
dc.contributor.author	Talledo, Miguel
dc.contributor.author	Pons, Maria J.
dc.contributor.author	Flores, Lídia
dc.contributor.author	Quispe, Ruth
dc.contributor.author	Ramírez, Pablo
dc.contributor.author	García de la Guarda, Ruth
dc.contributor.author	Alvarado, Débora
dc.contributor.author	Espinoza Culupú, Abraham
dc.contributor.author	Del Valle, Juana
dc.contributor.author	Vargas, Martha
dc.contributor.author	Ruiz, Joaquim
dc.date	2014
dc.identifier.citation	del Valle, LJ., Jaramillo, M., Talledo, M., Pons, M., Flores, L., Quispe, R., Ramírez, P., García, R., Alvarado, D., Espinoza, A., Del Valle, J., Vargas, M., Ruiz, J. Development of a 16S rRNA PCR-RFLP Assay for Bartonella identification: Applicability in the identification of species involved in human infections. "Universal Journal of Microbiology Research", 2014, vol. 2, núm. 1, p. 15-22.
dc.identifier.citation	2331-6721
dc.identifier.citation	10.13189/ujmr.2014.020102
dc.identifier.uri	http://hdl.handle.net/2117/102572
dc.language.iso	eng
dc.publisher	Society of Exploration Geophysicists (SEG)
dc.relation	www.hrpub.org
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	http://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.subject	Àrees temàtiques de la UPC::Enginyeria química
dc.subject	Bartonella
dc.subject	Genes
dc.subject	Bartonella
dc.subject	PCR-RFLP
dc.subject	16s rRNA Gene
dc.subject	Identification
dc.subject	Gens
dc.title	Development of a 16S rRNA PCR-RFLP Assay for Bartonella identification: Applicability in the identification of species involved in human infections
dc.type	info:eu-repo/semantics/submittedVersion
dc.type	info:eu-repo/semantics/article
dc.description.abstract	We designed a 16S rRNA gene PCR -RFLP scheme to identify all currently described Bartonella spp. The 16S rRNA genes of all Bartonella spp. were in-silico analyzed in order to design a RFLP technique able to discriminate among different species. The restriction enzymes selected were Mae III, Mse I, Sau96 I, Bsa AI, Drd I, Fok I, Bss HII, Bst UI, Alu I, Tsp DTI and Hph I which, according to a decision -making tree, faci litated the differentiation of all the currently described species of Bartonella .The technique was experimentally tested in different species of Bartonella , including human pathogenic B. bacilliformis and B. henselae with a 100% of concordance with the in-silico predicted patterns.This novel RFLP assay could be used to identify both human and non- human pathogenic Bartonella in diagnostic, phylogenetic and epidemiologic studies
dc.description.abstract	Peer Reviewed

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