dc.contributor |
Universitat de Barcelona |
dc.contributor.author |
Sindreu Balet, Carlos |
dc.contributor.author |
Bayés, Àlex |
dc.contributor.author |
Altafaj, Xavier |
dc.contributor.author |
Pérez Clausell, Jeús |
dc.date |
2017-03-27T10:04:05Z |
dc.date |
2017-03-27T10:04:05Z |
dc.date |
2014-03-07 |
dc.date |
2017-03-27T10:04:05Z |
dc.identifier.citation |
1756-6606 |
dc.identifier.citation |
633729 |
dc.identifier.uri |
http://hdl.handle.net/2445/108942 |
dc.format |
7 p. |
dc.format |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
BioMed Central |
dc.relation |
Reproducció del document publicat a: https://doi.org/10.1186/1756-6606-7-16 |
dc.relation |
Molecular Brain, 2014, vol. 7, num. 1, p. 16-22 |
dc.relation |
https://doi.org/10.1186/1756-6606-7-16 |
dc.rights |
cc-by (c) Sindreu Balet, Carlos et al., 2014 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.rights |
http://creativecommons.org/licenses/by/3.0/es |
dc.subject |
Zinc |
dc.subject |
Sinapsi |
dc.subject |
Neurotransmissors |
dc.subject |
Rates (Animals de laboratori) |
dc.subject |
Zinc |
dc.subject |
Synapses |
dc.subject |
Neurotransmitters |
dc.subject |
Rats as laboratory animals |
dc.title |
Zinc Transporter-1 Concentrates at the Postsynaptic Density of Hippocampal Synapses |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/publishedVersion |
dc.description.abstract |
BACKGROUND: Zinc concentrates at excitatory synapses, both at the postsynaptic density and in a subset of glutamatergic boutons. Zinc can modulate synaptic plasticity, memory formation and nociception by regulating transmitter receptors and signal transduction pathways. Also, intracellular zinc accumulation is a hallmark of degenerating neurons in several neurological disorders. To date, no single zinc extrusion mechanism has been directly localized to synapses. Based on the presence of a canonical PDZ I motif in the Zinc Transporter-1 protein (ZnT1), we hypothesized that ZnT1 may be targeted to synaptic compartments for local control of cytosolic zinc. Using our previously developed protocol for the co-localization of reactive zinc and synaptic proteins, we further asked if ZnT1 expression correlates with presynaptic zinc content in individual synapses. FINDINGS: Here we demonstrate that ZnT1 is a plasma membrane protein that is enriched in dendritic spines and in biochemically isolated synaptic membranes. Hippocampal CA1 synapses labelled by postembedding immunogold showed over a 5-fold increase in ZnT1 concentration at synaptic junctions compared with extrasynaptic membranes. Subsynaptic analysis revealed a peak ZnT1 density on the postsynaptic side of the synapse, < 10 nm away from the postsynaptic membrane. ZnT1 was found in the vast majority of excitatory synapses regardless of the presence of vesicular zinc in presynaptic boutons. CONCLUSIONS: Our study has identified ZnT1 as a novel postsynaptic density protein, and it may help elucidate the role of zinc homeostasis in synaptic function and disease. |