dc.contributor |
Universitat de Barcelona |
dc.contributor.author |
Zaouali, Mohamed Amine |
dc.contributor.author |
Panisello Roselló, Arnau |
dc.contributor.author |
Lopez, Alexandre |
dc.contributor.author |
Castro Benítez, Carlos |
dc.contributor.author |
Folch i Puy, Emma |
dc.contributor.author |
García Gil, Agustín |
dc.contributor.author |
Carbonell i Camós, Teresa |
dc.contributor.author |
Adam, R. (René) |
dc.contributor.author |
Roselló Catafau, Juan |
dc.date |
2018-03-20T15:12:53Z |
dc.date |
2018-03-20T15:12:53Z |
dc.date |
2017-06-21 |
dc.date |
2018-03-20T15:12:53Z |
dc.identifier.citation |
1007-9327 |
dc.identifier.citation |
671802 |
dc.identifier.uri |
http://hdl.handle.net/2445/120918 |
dc.format |
11 p. |
dc.format |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
Baishideng Publishing Group |
dc.relation |
Reproducció del document publicat a: https://doi.org/10.3748/wjg.v23.i23.4211 |
dc.relation |
World Journal of Gastroenterology, 2017, vol. 23, num. 23, p. 4211-4221 |
dc.relation |
https://doi.org/10.3748/wjg.v23.i23.4211 |
dc.rights |
cc-by-nc (c) Zaouali, Mohamed Amine et al., 2017 |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.rights |
http://creativecommons.org/licenses/by-nc/3.0/es |
dc.title |
Relevance of proteolysis and proteasome activation in fatty liver graft preservation: An Institut Georges Lopez-1 vs University of Wisconsin appraisal |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/publishedVersion |
dc.description.abstract |
AIM: To compare liver proteolysis and proteasome activation in steatotic liver grafts conserved in University of Wisconsin (UW) and Institut Georges Lopez-1 (IGL-1) solutions. METHODS: Fatty liver grafts from male obese Zücker rats were conserved in UW and IGL-1 solutions for 24 h at 4 °Cand subjected to "ex vivo" normo-thermic perfusion (2 h; 37 °C). Liver proteolysis in tissue specimens and perfusate was measured by reverse-phase high performance liquid chromatography. Total free amino acid release was correlated with the activation of the ubiquitin proteasome system (UPS: measured as chymotryptic-like activity and 20S and 19S proteasome), the prevention of liver injury (transaminases), mitochondrial injury (confocal microscopy) and inflammation markers (TNF 1 alpha, high mobility group box-1 (HGMB-1) and PPAR gamma), and liver apoptosis (TUNEL assay, cytochrome c and caspase 3). RESULTS:Profiles of free AA (alanine, proline, leucine, isoleucine, methionine, lysine, ornithine, and threonine, among others) were similar for tissue and reperfusion effluent. In all cases, the IGL-1 solution showed a significantly higher prevention of proteolysis than UW (P < 0.05) after cold ischemia reperfusion. Livers conserved in IGL-1 presented more effective prevention of ATP-breakdown and more inhibition of UPS activity (measured as chymotryptic-like activity). In addition, the prevention of liver proteolysis and UPS activation correlated with the prevention of liver injury (AST/ALT) and mitochondrial damage (revealed by confocal microscopy findings) as well as with the prevention of inflammatory markers (TNF1alpha and HMGB) after reperfusion. In addition, the liver grafts preserved in IGL-1 showed a significant decrease in liver apoptosis, as shown by TUNEL assay and the reduction of cytochrome c, caspase 3 and P62 levels. CONCLUSION: Our comparison of these two preservation solutions suggests that IGL-1 helps to prevent ATP breakdown more effectively than UW and subsequently achieves a higher UPS inhibition and reduced liver proteolysis. |