dc.contributor.author |
Gallego Palacios, Ana, 1987- |
dc.contributor.author |
Jáuregui, Olga |
dc.contributor.author |
Moyano Claramunt, Elisabet |
dc.contributor.author |
Palazón, Javier |
dc.contributor.author |
Casals, Isidre |
dc.contributor.author |
Bonfill Baldrich, Ma. Mercedes |
dc.date |
2015 |
dc.identifier.citation |
Gallego A, Jáuregui O, Moyano E, Palazón J, Casals I, Bonfill M. Optimization of a liquid chromatography-tandem mass spectrometry method for the quantification of traces of taxanes in a Corylus avellana cell suspension medium. /nRSC Advances. 2015; 5: 17976-17983. DOI 10.1039/C5RA00803D |
dc.identifier.citation |
2046-2069 |
dc.identifier.citation |
http://dx.doi.org/10.1039/C5RA00803D |
dc.identifier.uri |
http://hdl.handle.net/10230/26188 |
dc.format |
application/pdf |
dc.language.iso |
eng |
dc.publisher |
Royal Society of Chemistry |
dc.relation |
RSC Advances. 2015; 5: 17976-17983 |
dc.rights |
This article is licensed under a Creative Commons Attribution 3.0 Unported Licence. |
dc.rights |
info:eu-repo/semantics/openAccess |
dc.rights |
http://creativecommons.org/licenses/by/3.0/ |
dc.subject |
Avellaner |
dc.subject |
Plantes Cèl·lules i teixits |
dc.title |
Optimization of a liquid chromatography-tandem mass spectrometry method for the quantification of traces of taxanes in a Corylus avellana cell suspension medium |
dc.type |
info:eu-repo/semantics/article |
dc.type |
info:eu-repo/semantics/publishedVersion |
dc.description.abstract |
Since the recent discovery of taxol and other taxanes in Corylus avellana, this plant species has attracted interest as a potential new source of these compounds. However, its low taxane content in comparison with Taxus spp. has restricted research to analytical identification or global quantitation. A feasible and sensitive method based on liquid chromatography-tandem mass spectrometry using a triple quadrupole analyzer was developed for the analysis of taxol and four other taxanes in a Corylus avellana cell suspension medium. Taxanes were extracted from the cell culture medium with dichloromethane and analyzed using electrospray ionization and quantified by multiple-reaction monitoring mode. Methanol and matrix-matching calibration curves using docetaxel as the internal standard were analyzed. Linearity was confirmed over the whole calibration range (0.3–2.1 μg mL−1). The inter- and intra-day precision of taxanes ranged from 80% to 120% and the recovery rates were higher than 80%. Limits of detection were between 0.24–38 ng mL−1 and the limits of quantification were between 0.8–125 ng mL−1. The low detection and quantitation values obtained allowed us to detect small quantities of the released taxanes (120 ng mL−1 of B, 151 ng mL−1 of CF and 105 ng mL−1 of T), which correspond to about 0.5 ng mL−1 of each taxane, in the 20 mL Corylus avellana cell suspension culture medium extracted, even at the beginning of the culture. These results were confirmed by high resolution mass spectrometry. |
dc.description.abstract |
This research has been supported by a grant from the Spanish MEC (BIO2011-29856-CO2-01) and a grant from the Catalan Government (2014SGR215). A. Gallego was supported by a fellowship from the University Pompeu Fabra. |