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Título:	LC-MS/MS detection of unaltered glucuronoconjugated metabolites of metandienone
Autor/a:	Esquivel López, Argitxu; Pozo Mendoza, Óscar J., 1975-; Garrostas, Lorena; Balcells Aribau, Georgina, 1987-; Gómez Castellà, Cristina, 1985-; Kotronoulas, Aristotelis; Joglar Tamargo, Jesús; Ventura Alemany, Rosa
Abstract:	The aim of this study was to evaluate the direct detection of glucuronoconjugated metabolites of metandienone (MTD) and their detection times. Metabolites resistant to enzymatic hydrolysis were also evaluated. Based on the common mass spectrometric behaviour of steroid glucuronides, three liquid chromatography-tandem mass spectrometry (LC-MS/MS) strategies were applied for the detection of unpredicted and predicted metabolites: precursor ion scan (PI), neutral loss scan (NL), and theoretical selected reaction monitoring (SRM) methods. Samples from four excretion studies of MTD were analyzed for both the detection of metabolites and the establishment of their detection times. Using PI and NL methods, seven metabolites were observed in post-administration samples. SRM methods allowed for the detection of 13 glucuronide metabolites. The detection times, measured by analysis with an SRM method, were between 1 and 22 days. The metabolite detected for the longest time was 18-nor-17β-hydroxymethyl-17α-methyl-5β-androsta-1,4,13-triene-3-one-17-glucuronide. One metabolite was resistant to hydrolysis with β-glucuronidase; however it was only detected in urine up to four days after administration. The three glucuronide metabolites with the highest retrospectivity were identified by chemical synthesis or mass spectrometric data, and although they were previously reported, this is the first time that analytical data of the intact phase II metabolites are presented for some of them. The LC-MS/MS strategies applied have demonstrated to be useful for detecting glucuronoconjugated metabolites of MTD, including glucuronides resistant to enzymatic hydrolysis which cannot be detected by conventional approaches. Copyright © 2016 John Wiley & Sons, Ltd.
Abstract:	Grants by Ministerio de Economía y Competitividad (Gobierno de España) (Project number DEP2012-35612) and Generalitat deCatalunya (Consell Català de l’Esport and DIUE 2014 SGR 692) are gratefully acknowledged
Materia(s):	-Metabòlits -Esteroides
Derechos:	info:eu-repo/semantics/embargoedAccess This is the peer reviewed version of the following article: Esquivel A, Pozo OJ, Garrostas L, Balcells G, Gómez C, Kotronoulas A et al. LC-MS/MS detection of unaltered glucuronoconjugated metabolites of metandienone. Drug Test Anal. 2017 Apr; 9(4): 534-544, which has been published in final form at http://dx.doi.org/10.1002/dta.1996. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving
Tipo de documento:	Artículo Artículo - Versión aceptada
Editor:	Wiley
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