Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles

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dc.contributor.author	Olarte, Omar E.
dc.contributor.author	Licea-Rodriguez, Jacob
dc.contributor.author	Palero, Jonathan A.
dc.contributor.author	Gualda, Emilio J.
dc.contributor.author	Artigas, David
dc.contributor.author	Mayer, Jürgen, 1977-
dc.contributor.author	Swoger, Jim
dc.contributor.author	Sharpe, James
dc.contributor.author	Rocha-Mendoza, Israel
dc.contributor.author	Rangel-Rojo, Raul
dc.contributor.author	Loza-Alvarez, Pablo
dc.date	2012
dc.identifier.citation	Olarte OE, Licea-Rodriguez J, Palero JA, Gualda EJ, Artigas D, Mayer J et al. Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles. Biomed Opt Express. 2012 Jul 1; 3(7): 1492-505. DOI: 10.1364/BOE.3.001492
dc.identifier.citation	2156-7085
dc.identifier.citation	http://dx.doi.org/10.1364/BOE.3.001492
dc.identifier.uri	http://hdl.handle.net/10230/35196
dc.format	application/pdf
dc.language.iso	eng
dc.publisher	Optical Society of America (OSA)
dc.relation	info:eu-repo/grantAgreement/ES/3PN/TEC2009-09698
dc.relation	info:eu-repo/grantAgreement/ES/3PN/SAF2008-00211
dc.relation	info:eu-repo/grantAgreement/EC/FP7/217997
dc.relation	info:eu-repo/grantAgreement/EC/FP7/228933
dc.rights	© Oman E. Olarte et al 2012 Optical Society of America. Users may use, reuse, and build upon the article, or use the article for text or data mining, so long as such uses are for non-commercial purposes and appropriate attribution is maintained. All other rights are reserved
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	Feixos de làser
dc.subject	Microscòpia de fluorescència
dc.subject	Microscòpia
dc.subject	Processos multifotó
dc.title	Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles
dc.type	info:eu-repo/semantics/article
dc.type	info:eu-repo/semantics/publishedVersion
dc.description.abstract	We present the implementation of a combined digital scanned light-sheet microscope (DSLM) able to work in the linear and nonlinear regimes under either Gaussian or Bessel beam excitation schemes. A complete characterization of the setup is performed and a comparison of the performance of each DSLM imaging modality is presented using in vivoCaenorhabditis elegans samples. We found that the use of Bessel beam nonlinear excitation results in better image contrast over a wider field of view.
dc.description.abstract	This work is supported by the Generalitat de Catalunya grant 2009-SGR-159, the Spanish government grants TEC2009-09698 and SAF2008-00211, the EU project STELUM (FP7-PEOPLE-2007-3-1-IAPP, 217997), the Swiss National Science Foundation (SNSF) Sinergia project grant CRSII3_125447, the FP7-NMP VIBRANT project grant CP-IP 228933-2, the NoE P4L and Laserlab optobio. Jacob Licea-Rodriguez acknowledges CONACYT Mexico for supporting his stay at ICFO through a mixed fellowship. This research has been partially supported by Fundació Cellex Barcelona and was mainly conducted at ICFO’s “Super-Resolution Light Microscopy and Nanoscopy Facility”

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