Títol:
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Quantification of interacting cognate odorants with olfactory receptors in nanovesicles
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Autor/a:
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Sanmartí Espinal, Marta; Iavicoli, Patrizia; Calò, Annalisa; Taulés i Marín, Marta; Galve Bosch, Roger; Marco Colás, Ma. Pilar; Samitier i Martí, Josep
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Altres autors:
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Universitat de Barcelona |
Abstract:
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This study aims to improve our understanding of the interaction between olfactory receptors and odorants to develop highly selective biosensing devices. Natural nanovesicles (NVs) from Saccharomyces cerevisiae, ~100 nm in diameter, carrying either the human OR17-40 or the chimpanzee OR7D4 olfactory receptor (OR) tagged with the c-myc epitope at their N-terminus, are presented as model systems to quantify the interaction between odorant and olfactory receptors. The level of expression of olfactory receptors was determined at individual NVs using a novel competitive ELISA immunoassay comparing the values obtained against those from techniques involving the solubilization of cell membrane proteins and the identification of c-myc-carrying receptors. Surface Plasmon Resonance (SPR) measurements on L1 Biacore chips indicate that cognate odorants bind to their Ors, thereby quantifying the approximate number of odorants that interact with a given olfactory receptor. The selectivity of OR17-40-carrying NVs towards helional and OR7D4-carrying NVs towards androstenone has been proven in cross-check experiments with non-specific odorant molecules (heptanal and pentadecalactone, respectively) and in control receptors. |
Matèries:
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-Olfacte -Biosensors -Olors -Bioenginyeria -Smell -Biosensors -Odors -Bioengineering |
Drets:
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cc-by (c) Sanmartí Espinal, Marta et al., 2017
http://creativecommons.org/licenses/by/3.0/es |
Tipus de document:
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Article Article - Versió publicada |
Publicat per:
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Nature Publishing Group
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