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Título:	Different Storing and Processing Conditions of Human Lymphocytes do not Alter P-Glycoprotein Rhodamine 123 Efflux
Autor/a:	Chiva Blanch, Gemma; Giménez Bonafé, Pepita; Llaudó Vallmajor, Inés; Torras Ambròs, Joan; Cruzado, Josep Ma.; Petriz, Jordi; Castaño Boldú, Esther; Franquesa, Marcella; Herrero Fresneda, Immaculada; Tortosa i Moreno, Avelina; Rama, Inés; Bestard Matamoros, Oriol; Grinyó Boira, Josep M.; Lloberas Blanch, Núria
Abstract:	P-glycoprotein (Pgp), a protein codified by Multi Drug Resistance (MDR1) gene, has a detoxifying function and might influence the toxicity and pharmacokinetics and pharmacodynamics of drugs. Sampling strategies to improve Pgp studies could be useful to optimize the sensitivity and the reproducibility of efflux assays. This study aimed to compare Pgp expression and efflux activity by measuring Rhodamine123 (Rh123) retention in lymphocytes stored under different conditions, in order to evaluate the potential utility of any of the storing conditions in Pgp functionality. Our results show no change in protein expression of Pgp by confocal studies and Western blotting, nor changes at the mRNA level (qRT-PCR). No differences in Rh123 efflux by Pgp activity assays were found between fresh and frozen lymphocytes after 24 hours of blood extraction, using either of the two Pgp specific inhibitors (VP and PSC833). Different working conditions in the 24 hours post blood extraction do not affect Rh123 efflux. These results allow standardization of Pgp activity measurement in different individuals with different timing of blood sampling and in different geographic areas. _______________
Materia(s):	-Limfòcits -Glicoproteïnes -Toxicitat dels medicaments -Lymphocytes -Glycoproteins -Drug toxicity
Derechos:	cc-by-sa (c) Chiva Blanch, Gemma et al., 2009 http://creativecommons.org/licenses/by-sa/3.0/es
Tipo de documento:	Artículo Artículo - Versión publicada
Editor:	Canadian Society for Pharmaceutical Sciences
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