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Título:	Mapping forces and kinematics during collective cell migration
Autor/a:	Serra-Picamal, Xavier; Conte, Vito; Sunyer, Raimon; Muñoz Romero, José; Trepat, Xavier
Otros autores:	Universitat Politècnica de Catalunya. Departament de Matemàtiques; Universitat Politècnica de Catalunya. LACÀN - Mètodes Numèrics en Ciències Aplicades i Enginyeria
Abstract:	Fundamental biological processes including morphogenesis and tissue repair require cells to migrate collectively. In these processes, epithelial or endothelial cells move in a cooperative manner coupled by intercellular junctions. Ultimately, the movement of these multicellular systems occurs through the generation of cellular forces, exerted either on the substrate via focal adhesions (cell–substrate forces) or on neighboring cells through cell–cell junctions (cell–cell forces). Quantitative measurements of multicellular forces and kinematics with cellular or subcellular resolution have become possible only in recent years. In this chapter, we describe some of these techniques, which include particle image velocimetry to map cell velocities, traction force microscopy to map forces exerted by cells on the substrate, and monolayer stress microscopy to map forces within and between cells. We also describe experimental protocols to perform these measurements. The combination of these techniques with high-resolution imaging tools and molecular perturbations will lead to a better understanding of the mechanisms underlying collective cell migration in health and disease.
Abstract:	Peer Reviewed
Materia(s):	-Àrees temàtiques de la UPC::Enginyeria mecànica::Mecànica::Cinemàtica -Àrees temàtiques de la UPC::Enginyeria biomèdica::Biomecànica -Kinematics -Collective cell migration -Monolayer stress microscopy -Traction force microscopy -Cinemàtica -Bioenginyeria
Derechos:	http://creativecommons.org/licenses/by-nc-nd/3.0/es/
Tipo de documento:	Artículo - Versión presentada Artículo
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